LOCUS       sequence-105880-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses beta actin with both a PAGFP and mRFP tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Kueh HY, Charras GT, Mitchison TJ, Brieher WM
  TITLE     Actin disassembly by cofilin, coronin, and Aip1 occurs in bursts and
            is inhibited by barbed-end cappers.
  JOURNAL   J Cell Biol. 2008 Jul 28;182(2):341-53. doi: 10.1083/jcb.200801027.
  PUBMED    18663144
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2008-07-28- 28"; volume: "182"; 
            issue: "2"; pages: "341-53"
COMMENT     Sequence Label: Actin-mRFP-PAGFP
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(39..58)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     386..407
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
       61 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      121 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      181 acgtctatat catggccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
      241 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      301 gcgacggccc cgtgctgctg cccgacaacc actacctgag ccaccagtcc gccctgagca
      361 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      421 tcactctcgg catggacgag ctgtacaagg gcggcggcac tcgagctatg gatgatgata
      481 tcgccgcgct cgtcgtcgac aacggctccg gcatgtgcaa ggccggcttc gcgggcgacg
      541 atgccccccg ggccgtcttc ccctccatcg tggggcgccc caggcaccag ggcgtgatgg
      601 tgggcatggg tcagaaggat tcctatgtgg gcgacgaggc ccagagcaag agaggcatcc
      661 tcaccctgaa gtaccccatc gagcacggca tcgtcaccaa ctgggacgac atggagaaaa
      721 tctggcacca caccttctac aatgagctgc gtgtggctcc cgaggagcac cccgtgctgc
      781 tgaccgaggc ccccctgaac cccaaggcca accgcgagaa gatgacccag atcatgtttg
      841 agaccttc
//
