LOCUS       sequence-105883-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the FERM domain of Ezrin (N terminal portion of the 
            protein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Fritzsche M, Thorogate R, Charras G
  TITLE     Quantitative analysis of ezrin turnover dynamics in the actin 
            cortex.
  JOURNAL   Biophys J. 2014 Jan 21;106(2):343-53. doi: 
            10.1016/j.bpj.2013.11.4499.
  PUBMED    24461009
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2013.11"; journalName: "Biophys J"; date: 
            "2014-01-21- 21"; volume: "106"; issue: "2"; pages: "343-53"
COMMENT     Sequence Label: FERM domain of Ezrin
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(5..718,719..721,722..724))
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(48..68)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     663..691
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttcattattt gtagagctca tccatgccat gtgtaatccc agcagcagtt acaaactcaa
       61 gaaggaccat gtggtcacgc ttttcgttgg gatctttcga aagggcagat tgtgtcgaca
      121 ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat
      181 ggtctgctag ttgaacggat ccatcttcaa tgttgtggcg aattttgaag ttagctttga
      241 ttccattctt ttgtttgtct gccgtgatgt atacattgtg tgagttatag ttgtactcga
      301 gtttgtgtcc gagaatgttt ccatcttctt taaaatcaat accttttaac tcgatacgat
      361 taacaagggt atcaccttca aacttgactt cagcacgcgt cttgtagttc ccgtcatctt
      421 tgaaagatat agtgcgttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat
      481 gccgtttcat atgatccgga taacgggaaa agcattgaac accataagag aaagtagtga
      541 caagtgttgg ccatggaaca ggtagttttc cagtagtgca aataaattta agggtaagct
      601 ttccgtatgt agcatcacct tcaccctctc cactgacaga aaatttgtgc ccattaacat
      661 caccatctaa ttcaacaaga attgggacaa ctccagtgaa aagttcttct cctttgctag
      721 ccattctaga ctcgagcggc cgccactgtg ctggatatct gcagaattgc ccttgggcct
      781 gggccttcat ctgctgcacc tcgatggtgt caggcttcct gcggcgcata tacaactcat
      841 ggttgcccat gcagagctgc aggatccgct tgttgattct cagacgtggg gcataaaaca
      901 caaagtcagg tgccttcttg tcgatgggtt taatgacaaa ctttttgtca ttgaaagaga
      961 tgttcctgat ttcactccaa ggaaagccaa tctttggggt taacttatca tctttctcat
     1021 aaatattcag tccaagggca tcaactccaa gccaaaggtc tgttcctttc ttgtttttta
     1081 tctcgaaata
//
