LOCUS       sequence-105886-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of a germ-line targeted, codon optimized Cas9 for 
            genome-editing in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Moreno-Mateos MA, Vejnar CE, Beaudoin JD, Fernandez JP, Mis EK, 
            Khokha MK, Giraldez AJ
  TITLE     CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 
            targeting in vivo.
  JOURNAL   Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3543.
  PUBMED    26322839
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Aug 31. doi: 10.1038/nmeth.3543."
COMMENT     Sequence Label: pCS2-nCas9n-nanos 3'UTR
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      127..136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             145..165
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat ccgatccaat
      121 gtcgacgcca ccatggcttc tccacctaag aagaagagaa aggtgggaag catggataag
      181 aagtatagca tcggcctgga tattggaact aactccgtgg gttgggcagt gattacagac
      241 gactacaagg tccctagcaa gaaatttaag gtgctgggta acaccgacag gcacagcatc
      301 aagaaaaatc tgattggagc cctgctgttc ggttctggag agactgccga agcaacacgc
      361 ctgaaaagaa cagcaagaag gcgctatacc agaaggaaga atagaatctg ttacctgcag
      421 gagattttct ctaacgaaat ggctaaggtg gacgattcat tctttcatag gctggaggaa
      481 agtttcctgg tcgaggaaga taagaaacac gagcgccatc ctatctttgg aaacattgtg
      541 gacgaggtcg cctatcacga aaaataccca accatctatc atctgcgcaa gaaactggct
      601 gactctactg ataaagccga cctgagactg atctatctgg ctctggccca catgattaag
      661 ttcaggggtc attttctgat cgagggcgat ctgaaccccg acaattccga tgtggacaag
      721 ctgttcatcc agctggtcca gatttacaat cagctgtttg aggaaaaccc tattaatgct
      781 tccagagtgg acgcaaaagc tatcctgtca gccaggctgt ccaagtcacg cagactggag
      841 aacctgattg cacagctgcc cggagaaaag aggaacggtc tgtttggaaa tctgatcgct
      901 ctgagtctgg gcctgactcc taacttcaaa agcaattttg atctggctga ggacgccaaa
      961 ctgcagctgt caaaggacac atatgacgat gacctggata acctgctggc acagatcgga
     1021 gatcagtacg ctgacctgtt cctggctgcc aaaaatctgt ccgacgcaat cctgctgtca
     1081 gatattctga
//
