LOCUS       sequence-105887-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of a germ-line targeted, codon optimized Cas9 for 
            genome-editing in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Moreno-Mateos MA, Vejnar CE, Beaudoin JD, Fernandez JP, Mis EK, 
            Khokha MK, Giraldez AJ
  TITLE     CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 
            targeting in vivo.
  JOURNAL   Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3543.
  PUBMED    26322839
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Aug 31. doi: 10.1038/nmeth.3543."
COMMENT     Sequence Label: pCS2-nCas9n-nanos 3'UTR
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttgtccagtt ctgacagtcc accgcgctct gcttttgtca ggttatcaaa cttcctctga
       61 gtgatcagtt tggcattcag cagctgtctc cagtagttct tcattttctt gaccacttcc
      121 tcactaggga cattatctga ttttcctctg tttttgtcgg acctggtcag caccttgttg
      181 tcaatgctgt catctttgat gaaactctgg gggacgatat gatccacgtc gtaatcagac
      241 agcctgttaa tatccagctc ctggtccacg tacatatctc ttccattctg cagatagtac
      301 agatacagct tctcattctg cagctgtgtg ttttccactg ggtgctcttt caggatctgg
      361 ctacccagct ccttaatgcc ttcctcgatt cttttcatcc tctctctact gttcttctga
      421 cccttctggg ttgtctgatt ttcgcgagcc atctcaatca cgatgttttc gggcttatgg
      481 cccatgactt tcaccagctc atcgacgatt ttcactgtct gcagaatgcc tttcttgatg
      541 gctgggcttc cagccaggtt tgcaatctgc tcatgcaggg agtggccctg tccagacacc
      601 tgagccttct ggatgtcctc tttgaaggtc agactgtcat cgtgaatcag ctgcataaaa
      661 ttgcggttgg cgaagccatc acttttcaga aagtccagga ttgtctttcc tgactgttta
      721 tccctaatgc cgttgatcag cttcctgctc agtctacccc agccagtgta gcgccttctc
      781 ttcagctgtt tcatcacctt gtcatcgaac agatgagcat aggttttcag tctttcctcg
      841 atcattcctc tgtcctcaaa cagggtcagt gtcagcacaa tgtcttccag gatatcctca
      901 ttttcctcgt tgtccaggaa gtctttatcc ttaatgatct tcagcagatc gtggtaagcg
      961 cccaggcttg cgttaaatct gtcttcgac
//
