LOCUS       sequence-105888-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains FLAG epitope for immunofluorescence detection of resorufin 
            ligase for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Liu DS, Nivon LG, Richter F, Goldman PJ, Deerinck TJ, Yao JZ, 
            Richardson D, Phipps WS, Ye AZ, Ellisman MH, Drennan CL, Baker D, 
            Ting AY
  TITLE     Computational design of a red fluorophore ligase for site-specific 
            protein labeling in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Oct 28;111(43):E4551-9. doi: 
            10.1073/pnas.1404736111. Epub 2014 Oct 13.
  PUBMED    25313043
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404736111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-10-28- 28"; volume: "111"; issue: "43"; pages: "E4551-9"
COMMENT     Sequence Label: pcDNA3-FLAG-LplA(AAG)
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             120..137
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             141..173
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(177..185,186..200)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             207..230
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttgcggccgc caccatggtg cggggttctc
      121 atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg ggtcgggatc
      181 tgtacgacga tgacgataag gatccagact acaaggatga cgacgataag atgagcaccc
      241 tgagactgct gatcagcgac agctacgacc cctggttcaa cctggccgtg gccgagtgca
      301 tcttcagaca gatgcccgcc acccagagag tgctgttcct gtggagaaac gccgacaccg
      361 tggtgatcgg cagagcccag aacccctgga aggagtgcaa caccagaaga atggaggagg
      421 acaacgtgag actggccaga agaagcagcg gcggcggcgc cgtgttccac gacctgggca
      481 acacctgctt caccttcatg gccggcaagc ccgagtacga caagaccatc agcaccagca
      541 tcgtgctgaa cgccctgaac gccctgggcg tgagcgccga ggccagcggc agaaacgacc
      601 tggtggtgaa gaccgtggag ggcgacagaa aggtgagcgg cagcgcctac agagagacca
      661 aggacagagg cgcccacggc ggcaccctgc tgctgaacgc cgacctgagc agactggcca
      721 actacctgaa ccccgacaag aagaagctgg ccgccaaggg catcaccagc gtgagaagca
      781 gagtgaccaa cctgaccgag ctgctgcccg gcatcaccca cgagcaggtg tgcgaggcca
      841 tcaccgaggc cttcttcgcc cactacggcg agagagtgga ggccgagatc atcagcccca
      901 acaagacccc cgacctgccc aacttcgccg agaccttcgc caga
//
