LOCUS       sequence-105890-        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains FLAG epitope for immunofluorescence detection of resorufin 
            ligase for mammalian cell expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Liu DS, Nivon LG, Richter F, Goldman PJ, Deerinck TJ, Yao JZ, 
            Richardson D, Phipps WS, Ye AZ, Ellisman MH, Drennan CL, Baker D, 
            Ting AY
  TITLE     Computational design of a red fluorophore ligase for site-specific 
            protein labeling in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Oct 28;111(43):E4551-9. doi: 
            10.1073/pnas.1404736111. Epub 2014 Oct 13.
  PUBMED    25313043
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404736111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-10-28- 28"; volume: "111"; issue: "43"; pages: "E4551-9"
COMMENT     Sequence Label: pcDNA3-FLAG-LplA(AAG)
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        3..21
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3..20
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gcatttaggt gacactatag aatagggccc tctagatgca tgctcgagcg gccgccagtg
       61 tgatggatat ctgcagaatt cttatctcac ggcgccggcc atccaggcgc tcagctctct
      121 cagctccttc tcctgctcgg ggaagtccac cagcagggcc tcgcactcct gctgcagcat
      181 gtcggctctg tacaggcagc cctgcagtct gccggccagg gcctccaggg gggcggggtt
      241 caggctgtcg gtgaacacct gggctctggt gatgtggccc ttctccacgt cgaagtgcag
      301 ctccacgccg ccccaggtga atctctcgtc cagcaggtgg ctgaaggcgg gggcctggcc
      361 gaagttccac tcccagctgc tctgtctggc gaaggtctcg gcgaagttgg gcaggtcggg
      421 ggtcttgttg gggctgatga tctcggcctc cactctctcg ccgtagtggg cgaagaaggc
      481 ctcggtgatg gcctcgcaca cctgctcgtg ggtgatgccg ggcagcagct cggtcaggtt
      541 ggtcactctg cttctcacgc tggtgatgcc cttggcggcc agcttcttct tgtcggggtt
      601 caggtagttg gccagtctgc tcaggtcggc gttcagcagc agggtgccgc cgtgggcgcc
      661 tctgtccttg gtctctctgt aggcgctgcc gctcaccttt ctgtcgccct ccacggtctt
      721 caccaccagg tcgtttctgc cgctggcctc ggcgctcacg cccagggcgt tcagggcgtt
      781 cagcacgatg ctggtgctga tggtcttgtc gtactcgggc ttgccggcca tgaaggtgaa
      841 gcaggtgttg cccaggtcgt ggaacacggc gccgccgccg ctgcttcttc tggccagtct
      901 cacgttgtcc tcctccattc ttctggtgtt gcactccttc caggggttct gggctctgcc
      961 gatcaccacg gtgtcggcgt ttctccacag gaacagcact ctctgggtgg cgggcatctg
     1021 tctgaagatg cactcggcca cggccaggtt gaaccagggg tcgtagctgt cgctgatcag
     1081 cagt
//
