LOCUS       sequence-105892-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains GFP tag for direct live-cell fluorescence imaging of the 
            resofurin ligase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Liu DS, Nivon LG, Richter F, Goldman PJ, Deerinck TJ, Yao JZ, 
            Richardson D, Phipps WS, Ye AZ, Ellisman MH, Drennan CL, Baker D, 
            Ting AY
  TITLE     Computational design of a red fluorophore ligase for site-specific 
            protein labeling in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Oct 28;111(43):E4551-9. doi: 
            10.1073/pnas.1404736111. Epub 2014 Oct 13.
  PUBMED    25313043
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404736111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-10-28- 28"; volume: "111"; issue: "43"; pages: "E4551-9"
COMMENT     Sequence Label: pcDNA3-GFP-LplA(AAG)
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        3..21
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3..20
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gcatttaggt gacactatag aatagggccc tctagagggc atcgttccca ctgcgatgct
       61 ggttgccaac gatcagatgg cgttgggcgc aatgcgcgcc attaccgagt ccgggctgcg
      121 cgttggtgcg ctcgagcggc cgccagtgtg atggatatct gcagaattct tatctcacgg
      181 cgccggccat ccaggcgctc agctctctca gctccttctc ctgctcgggg aagtccacca
      241 gcagggcctc gcactcctgc tgcagcatgt cggctctgta caggcagccc tgcagtctgc
      301 cggccagggc ctccaggggg gcggggttca ggctgtcggt gaacacctgg gctctggtga
      361 tgtggccctt ctccacgtcg aagtgcagct ccacgccgcc ccaggtgaat ctctcgtcca
      421 gcaggtggct gaaggcgggg gcctggccga agttccactc ccagctgctc tgtctggcga
      481 aggtctcggc gaagttgggc aggtcggggg tcttgttggg gctgatgatc tcggcctcca
      541 ctctctcgcc gtagtgggcg aagaaggcct cggtgatggc ctcgcacacc tgctcgtggg
      601 tgatgccggg cagcagctcg gtcaggttgg tcactctgct tctcacgctg gtgatgccct
      661 tggcggccag cttcttcttg tcggggttca ggtagttggc cagtctgctc aggtcggcgt
      721 tcagcagcag ggtgccgccg tgggcgcctc tgtccttggt ctctctgtag gcgctgccgc
      781 tcacctttct gtcgccctcc acggtcttca ccaccaggtc gtttctgccg ctggcctcgg
      841 cgctcacgcc cagggcgttc agggcgttca gcacgatgct ggtgctgatg gtcttgtcgt
      901 actcgggctt gccggccatg aaggtgaagc aggtgttgcc caggtcgtgg aacacggcgc
      961 cgccgccgc
//
