LOCUS       sequence-105920-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SP-Cas9 protein in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V,
            Lebbink RJ, Rehmann H, Geijsen N
  TITLE     Efficient Intracellular Delivery of Native Proteins.
  JOURNAL   Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028.
  PUBMED    25910214
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.03"; journalName: "Cell"; date: "2015-04-23- 
            23"; volume: "161"; issue: "3"; pages: "674-690"
COMMENT     Sequence Label: SP-Cas9
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    79..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             118..140
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
      121 gtttaacttt aagaaggaga tataccatgg ataaaaaata cagcattggt ctggacattg
      181 gcacgaatag cgttggttgg gcagtgatta ccgatgaata caaagtcccg tcgaaaaaat
      241 tcaaagtgct gggtaacacc gatcgccata gcattaagaa aaacctgatc ggtgcgctgc
      301 tgtttgattc tggcgaaacc gcggaagcaa cgcgtctgaa acgtaccgca cgtcgccgtt
      361 acacgcgccg taaaaatcgt atttgctatc tgcaggaaat ctttagcaac gaaatggcga
      421 aagtcgatga ctcatttttc caccgcctgg aagaatcgtt tctggtggaa gaagataaaa
      481 aacatgaacg tcacccgatt ttcggcaata tcgttgatga agtcgcgtac catgaaaaat
      541 atccgacgat ttaccacctg cgtaaaaaac tggtggattc taccgacaaa gccgatctgc
      601 gcctgattta tctggcactg gctcatatga tcaaatttcg tggtcacttc ctgattgaag
      661 gcgacctgaa cccggataat agtgacgtcg ataaactgtt tattcagctg gtgcaaacct
      721 ataatcagct gttcgaagaa aacccgatca atgcaagtgg tgttgatgcg aaagccattc
      781 tgtccgctcg cctgagtaaa tcccgccgtc tggaaaacct gattgcacag ctgccgggtg
      841 aaaagaaaaa cggtctgttt ggcaatctga tcgctctgtc actgggcctg acgccgaact
      901 ttaaatcgaa tttcgacctg gcaga
//
