LOCUS       sequence-105925-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SP-Cas9 protein in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V,
            Lebbink RJ, Rehmann H, Geijsen N
  TITLE     Efficient Intracellular Delivery of Native Proteins.
  JOURNAL   Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028.
  PUBMED    25910214
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.03"; journalName: "Cell"; date: "2015-04-23- 
            23"; volume: "161"; issue: "3"; pages: "674-690"
COMMENT     Sequence Label: SP-Cas9
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(65..85)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttcctttcgg gctttgttag cagccggatc cttagtgatg atgatgatga tgcgagccac
       61 cgcccacttt gcgtttcttt ttcggcagac gggtgccgga gccaccgccg tcaccgccca
      121 gctgactcag atcaatacgc gtttcataca gaccggtgat cgattgatga atcagggtag
      181 catccagaac ttctttcgtt gaggtatagc gtttacggtc gatcgtggta tcgaagtatt
      241 taaaagctgc cggtgcgccc aggttcgtca gggtaaacag gtggataata ttttcggcct
      301 gttcgcgaat cggtttatca cgatgtttgt tatacgcgct cagaactttg tccagattgg
      361 catccgccag aatcacgcgt ttcgagaatt ctgagatctg ttcgatgatt tcatccaggt
      421 agtgtttatg ttgttcgacg aacagttgtt tctgttcgtt atcttccgga gaacctttca
      481 gtttttcata atggctagcc aggtacagaa agttaacata tttactcggc agtgccagtt
      541 cattgccttt ctgcagttca ccagcagatg ccagcatgcg tttacggccg ttttccagtt
      601 cgaacaggct atatttcggc agtttgatga tcagatcttt tttcacttct ttgtaacctt
      661 tggcttccag gaagtcaatc gggtttttct caaaagagct acgttccatg atggtaatgc
      721 ccagcagttc tttcacggat ttcagttttt tacttttacc tttttcgact ttagccacga
      781 ccaggacgga atatgcaacc gtcggactat caaaaccgcc gtattttttc gggtcccaat
      841 cttttttgcg cgcaatcagt ttatcgctgt tacgtttcgg caggatggat tctttactaa
      901 aaccgcccgt ctgcacttcg gttttcttca cgatattcac ttgcggcatc gacaggactt
      961 tgcgaacggt cgcgaagtca cggcctttat cccacacaat ttcgcc
//
