LOCUS       sequence-105930-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_5
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(117..151)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     206..223
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(247..304)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(332..359)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(375..446)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaaggcctgc agcaaacgac gaaaactacg cttaagcacc gaccgcagcc acagcgtgta
       61 gcgctcccag gccgtccact ccctgatccg gcgctcgtta tcgctctcat cgaactgcta
      121 gcattatacc taggactgag ctagctgtca aattaccgcc tttgagtgag ctgataccgc
      181 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcct gcataacgcg
      241 aagtaatctt ttcggtttta aagaaaaagg gcagggtggt gacaccttgc ccttttttgc
      301 cggactgcag ctgccgctac tagtatataa acgcagaaag gcccacccga aggtgagcca
      361 gtgtgactct agtagagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
      421 ttcgactgag cctttcgttt tatttgatgc ctggctctag tattattaag caccggtgga
      481 gtgacgacct tcagcacgtt cgtactgttc aacgatggtg tagtcttcgt tgtgggaggt
      541 gatgtccagt ttgatgtcgg ttttgtaagc acccggcagc tgaaccggtt ttttagccat
      601 gtaggtggtt ttaacttcag cgtcgtagtg accaccgtct ttcagtttca gacgcatttt
      661 gatttcacct ttcagagcac cgtcttccgg gtacatacgt tcggtggaag cttcccaacc
      721 catggttttt ttctgcataa ccggaccgtc ggacgggaag ttggtaccac gcagtttaac
      781 tttgtagatg aactcaccgt cttgcaggga ggagtcctgg
//
