LOCUS       sequence-105933-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  It is a donor vector for generation of recombinant baculovirus by 
            site-specific transposition in an E. coli host..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  TITLE     Arrowsmith Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFBOH-MHL
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             68..85
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             98..118
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(146..179)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        194..639
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc tcggtccgaa
       61 aaccatgcat catcatcatc atcacagcag cggcagagaa aacttgtatt tccagggcca
      121 tatgagttct cctcctgaaa gatccataac ttcgtatagc atacattata cgaagttatg
      181 cggccgcgac gtccacatat acctgccgtt cactattatt tagtgaaatg agatattatg
      241 atattttctg aattgtgatt aaaaaggcaa ctttatgccc atgcaacaga aactataaaa
      301 aatacagaga atgaaaagaa acagatagat tttttagttc tttaggcccg tagtctgcaa
      361 atccttttat gattttctat caaacaaaag aggaaaatag accagttgca atccaaacga
      421 gagtctaata gaatgaggtc gaaaagtaaa tcgcgcgggt ttgttactga taaagcaggc
      481 aagacctaaa atgtgtaaag ggcaaagtgt atactttggc gtcacccctt acatatttta
      541 ggtctttttt tattgtgcgt aactaacttg ccatcttcaa acaggagggc tggaagaagc
      601 agaccgctaa cacagtacat aaaaaaggag acatgaacga tgaacatcaa aaagtttgca
      661 aaacaagcaa cagtattaac ctttactacc gcactgctgg ca
//
