LOCUS       sequence-105935-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of A5NT sgRNA from the arabinose-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-PBAD-sgRNA-A5NT
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt
       61 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct
      121 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc
      181 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat
      241 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat
      301 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa
      361 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt
      421 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat
      481 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa
      541 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg
      601 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca
      661 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga
      721 ta
//
