LOCUS       sequence-105963-        7362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This is a 3rd generation lentiviral transfer vector (highest titer) 
            from Nolan lab's FELIX vector system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7362)
  TITLE     Nolan Lab Plasmids
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTiger
FEATURES             Location/Qualifiers
     source          1..7362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    403..991
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(456..476)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(874..885)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(994..1010)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(995..1011)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1302..1318)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1302..1318)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1315..1337)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1326..1342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1350..1356,1357..1374,1375..1380))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1395..1416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1533..1550)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1704..2292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(2463..3254,3255..3323))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3086..3105
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3324..3428)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3496..3514
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3552..3574)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3674..3693
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3847..3868)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        4396..4775
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4776..4979
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        6039..6057
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        6103..6482
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6483..6686
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        6731..6749
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttaaaggga ctattgggag gtatcttggg aataggatta ggagtgttat tattgatttt
       61 atgtttacct acattggttg attgtataag aaattgtatc cacaagatac taggatacac
      121 agtaattgca atgcctgaag tagaaggaga agaaatacaa ccacaaatgg aattgaggag
      181 aaatggtagg caatgtggca tgtctgaaaa agaggaggaa tgatgaagta tctcagactt
      241 attttataag ggagatactg tgctgagttc ttccctttga ggaaggtatg tcatatgaat
      301 ccatttcgaa tcaaatcaaa ctaataaagt atgtattgta aggtaaaagg aaaagacaaa
      361 gaagaagaag aaagaagaaa gccttcaatc aagcttatcg ataatcaacc tctggattac
      421 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
      481 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
      541 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
      601 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
      661 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
      721 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
      781 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
      841 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
      901 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
      961 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggggggcc
     1021 cctgaagatg aacctcctaa aaaagaaaaa agggtggact gggatgagta ttggaaccct
     1081 gaaggcctga ttgatcagct cctaggtaac cagtgctttg tgaaacttcg aggagtctct
     1141 ttgttgagga cttttgagtt ctcccttgag gctcccacag atacaataaa tatttgagat
     1201 tgaaccctgt cgagtatctg tgtaatcttt tttacctgtg aggtctcgga atccgggccg
     1261 agaacttcgc actgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     1321 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1381 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1441 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1501 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1561 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1621 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1681 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1741 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1801 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1861 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1921 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1981 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2041 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2101 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2161 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2221 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2281 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2341 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2401 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2461 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2521 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2581 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2641 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2701 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2761 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2821 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2881 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2941 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3001 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3061 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3121 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3181 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3241 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3301 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3421 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     3481 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     3541 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     3601 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     3661 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccataa aattgtaaac
     3721 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3781 tagaccgaaa tcggcaaaat cccttataaa tcaaaagaat agcccgagat agagttgagt
     3841 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3901 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt
     3961 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     4021 gcttgacggg gaaagccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4081 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4141 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4201 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatggctcg acagatcttc
     4261 aatattggcc attagccata ttattcattg gttatatagc ataaatcaat attggctatt
     4321 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     4381 tatgaccgcc atgttggcat tgattattga ctagttatta atagtaatca attacggggt
     4441 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     4501 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     4561 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     4621 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
     4681 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
     4741 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
     4801 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     4861 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg
     4921 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     4981 tgtgaaactt cgaggagtct ctttgttgag gacttttgag ttctcccttg aggctcccac
     5041 agatacaata aatatttgag attgaaccct gtcgagtatc tgtgtaatct tttttacctg
     5101 tgaggtctcg gaatccgggc cgagaacttc gcagttggcg cccgaacagg gacttgattg
     5161 agagtgattg aggaagtgaa gctagagcaa tagaaagctg ttaagcagaa ctcctgctga
     5221 cctaaatagg gaagcagtag cagacgctgc taacagtgag tatctctagt gaagcagact
     5281 cgagctcata atcaagtcat tgtttaaagg cccagataaa ttacatctgg tgactcttcg
     5341 cggaccttca agccaggaga ttcgccgagg gacagtcaac aaggtaggag agattctaca
     5401 gcaacatggg gaatggacag gggcgagatt ggaaaatggc cattaagaga tgtagtaatg
     5461 ttgctgtagg agtagggggg aagagtaaaa aatttggaga agggaatttc agatgggcca
     5521 ttagaatggc taatgtatct acaggacgag aacctggtga tataccagag actttagatc
     5581 aactaaggtt ggttatttgc gatttacaag aaagaagaga aaaatttgga tctagcaaag
     5641 aaattgatat ggcaattact taaggactga atttaattta cctaagatgg tagcagaaga
     5701 gataagacga aaatgcccag tatgcagaat cagaggagaa caagtgggag gacaattgaa
     5761 aatagggcct ggtatctggc aaatggattg cacacacttt gatggcaaaa taattcttgt
     5821 gggtaactcc ttccatcttt tgatttttaa aatttggtcc attatctgtt tgtaattcag
     5881 taacattatg agcactcaac aattgtaaaa cagctttaac tgtacagtca gcagtttctt
     5941 gagaaattat ttgtgcccat atatatcctg attccacatg taaattgtga cattaaaagt
     6001 ctttgcggta gcaggacttt taaatatgac ggcgcgcgta atacgactca ctatagggcg
     6061 aattgggtac cgggcccgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     6121 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     6181 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     6241 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     6301 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     6361 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     6421 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     6481 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     6541 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     6601 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     6661 ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact gcttactggc
     6721 ttatcgaaat taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa
     6781 gcttggtacc gagctcggat ccactagtcc agtgtggtgg aattctgcag atatccagca
     6841 cagtggcggc cgccagtgtg atggatatct gcagaattca ggcctaggag gataggaggg
     6901 atggcccctt atgaattatt agcacaacaa gaatccttaa gaatacaaga ttatttttct
     6961 gcaataccac aaaaattgca cgtgcctgaa ttccagcaca ctggcggccg ctcgagtcta
     7021 gcgcgcctaa tagaagctag taataggatt aaaagtagaa gctatggaaa aatttttata
     7081 tacagctttc gctatgcaag aattaggatg taatcaaaat caattcttct gcaaaatccc
     7141 tcttgagttg tggacaaggt ataatatgac tataaatcaa acaatatgga atcatggaaa
     7201 tataactttg ggggaatggt ataaccaaac aaaagattta caacaaaagt tttatgaaat
     7261 aataatggac atagaacaaa ataatgtaca agggaaaaca gggatacaac aattacaaaa
     7321 gtgggaagat tgggtaagat ggataggaaa tattccacaa ta
//
