LOCUS       sequence-105989-        7776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human beta-catenin with Flag tag and S33Y 
            mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Kolligs FT, Hu G, Dang CV, Fearon ER
  TITLE     Neoplastic transformation of RK3E by mutant beta-catenin requires 
            deregulation of Tcf/Lef transcription but not activation of c-myc 
            expression.
  JOURNAL   Mol Cell Biol. 1999 Aug . 19(8):5696-706.
  PUBMED    10409758
REFERENCE   2  (bases 1 to 7776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Aug . 19(8):5696-706."
COMMENT     Sequence Label: pcDNA3-S33Y Beta-catenin
FEATURES             Location/Qualifiers
     source          1..7776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2268..2287)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2269..2287)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2332..2535)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2361..2381)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2536..2915
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3087..3106
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3181..3285
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3286..3354,3355..4146)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3504..3523)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4317..4905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4806..4825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5059..5076
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5193..5214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5229..5234,5235..5252,5253..5259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5267..5283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5272..5294
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5291..5307
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5291..5307
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5356..5477
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5367..5386)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5421..5440
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5651..6445)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5762..5781)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6372..6391
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6512..6841)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6526..6661
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6580..6599)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6826..6846
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(6855..7283)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6965..6986)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7177..7196
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    7329..7553
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7542..7559
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        7579..7597
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7579..7596
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(7635..7658)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(join(7659..7776,1..2225))
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDYGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     CDS             complement(join(7659..7776,1..2225))
                     /codon_start=1
                     /gene="Homo sapiens CTNNB1"
                     /product="human cadherin-associated protein that also plays
                     a role in the canonical Wnt signaling pathway"
                     /label=beta-catenin
                     /note="also known as catenin beta-1"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDYGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
ORIGIN
        1 tggccaccca tctcatgttc catcatgggg tccataccca aggcatcctg gccatatcca
       61 ccagagtgaa aagaacgata gctaggatca tcctggcgat atccaagggg ttctccctgg
      121 gcaccaatat caagtccaag atcagcagtc tcattccaag ccattggctc tgttctgaag
      181 agagagctgg tcagctcaac tgaaagccgt ttcttgtaat cttgtggctt gtcctcagac
      241 attcggaaca aaacagcagc tgcatatgtc gccacacctt cattcctaga gtgaagtaac
      301 tctgtcagag gagctgtggc tccctcagct tcaatagctt ctgcagcttc cttgtcctga
      361 gcaagttcac agaggacccc tgcagctact ctttggatgt tttcaatggg agaataaagc
      421 agctgcacaa acaatggaat ggtatttagt cctctgataa caattcggtt gtgaacatcc
      481 cgagctagga tgtgaagggc tccggtacaa ccttcaacta tttcttccat gcggaccccc
      541 tccacaaatt gctgctgtgt cccacccatg gacgtacggc gctgggtatc ctgatgtgca
      601 cgaacaagca actgaactag tcgtggaatg gcaccctgct cacgcaaagg tgcatgattt
      661 gcgggacaaa gggcaagatt tcgaatcaat ccaacagtag cctttatcag aggccagtgg
      721 gatggtgggt gtaagagctt aaccacaact ggtagtccat agtgaaggcg aactgcattc
      781 tgggccatct ctgcttcttg gtgtcggctg gtcagatgac gaagagcaca gatggcaggc
      841 tcagtgatgt cttccctgtc accagcccga aggacagtac gcacaagagc ctctatacca
      901 cccacttggc agaccatcat cttgttctta taattattgc aagtgaggtt agaaagaatt
      961 ccagctgcac aggtgaccac atttatatca tctgaaccca gaagctgaac aagagtccca
     1021 aggagacctt ccatcccttc ctgtttagtt gcagcatctg aaagattcct gagagtccaa
     1081 agacagttct gaacaagacg ttgacttgga tctgtcaggt gaagtcctaa agcttgcatt
     1141 ccaccagctt ctacaatagc cggcttatta ctagagcaga cagatagcac cttcagcact
     1201 ctgcttgtgg tccacagtag tttttcgtaa gtataggtcc tcattatatt tactaaagct
     1261 tggggtccac cactagccag tatgatgagc ttgctttctt ggttgccata agctaaaatt
     1321 tgaaggcagt ctgtcgtaat agccaagaat ttaacatttg ttttgttgag caaggcaacc
     1381 attttctgca gcccaccagc taaacgcact gccattttag ctccttcttg atgtaataaa
     1441 aggttgtgga gagttgtaat ggcataaaac aacacagaat ccactggtga accaagcatt
     1501 ttcaccaggg caggaatgcc tccagactta aagatggcca gtaagccctc acgatgatgg
     1561 gaaaggttat gcaaggtccc agcggtacaa cgagctgttt ctacatcatt tgtattctgc
     1621 atggtacgta caatagcaga caccatctga ggagaacgca tgatagcgtg tctggaagct
     1681 tcctttttag aaagctgatg gaccataact gcagccttat taaccaccac ctggtcctcg
     1741 tcatttagca gttttgtcag ttcagggatt gcacgtgtgg caagttctgc atcatcttga
     1801 tagttaatca agtttacaac tgcatgtttc agcatctgtg atggttcagc caaacgctgg
     1861 acattagtgg gatgagcagc atcaaactgt gtagatggga tctgcatgcc ctcatctaat
     1921 gtctcaggga acatagcagc tcgtaccctc tgagctcgag tcattgcata ctgtccatca
     1981 atatcagcta cttgttcttg agtgaaggac tgagaaaatc cctgttccca ctcatacagg
     2041 acttgggagg tatccacatc ctcttcctca ggattgcctt taccactcag agaaggagct
     2101 gtggtagtgg caccagaatg gattccatag tccaggtaag actgttgctg ccagtgacta
     2161 acagccgctt ttctgtctgg ttccatggcc atgtccaact ccatcaaatc agcttgagta
     2221 gccattgtcc acgctgggga tccgagctcg gtaccaagct tgggtctccc tatagtgagt
     2281 cgtattaatt tcgataagcc agtaagcagt gggttctcta gttagccaga gagctctgct
     2341 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
     2401 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
     2461 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
     2521 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     2581 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     2641 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     2701 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     2761 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     2821 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     2881 tgattactat taataactag tcaataatca atgtcaacgc gtatatctgg cccgtacatc
     2941 gcgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattgt cggtcaagcc
     3001 ttgccttgtt gtagcttaaa ttttgctcgc gcactactca gcgacctcca acacacaagc
     3061 agggagcaga tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     3121 taggggatcg ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg
     3181 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3241 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     3301 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3361 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3421 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3481 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3541 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3601 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3661 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3721 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3781 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     3841 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     3901 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3961 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4021 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4081 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4141 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     4201 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4261 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4321 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4381 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4441 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     4501 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4561 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4621 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4681 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4741 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4801 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4861 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4921 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4981 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5041 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     5101 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     5161 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     5221 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     5281 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     5341 ggtatacaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     5401 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     5461 gctgcaataa acaagttggg gtgggcgaag aactccagca tgagatcccc gcgctggagg
     5521 atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg
     5581 gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc
     5641 agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg
     5701 gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag
     5761 caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac
     5821 agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc
     5881 catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt
     5941 cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt
     6001 ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag
     6061 ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag
     6121 gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc
     6181 ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc
     6241 acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga
     6301 caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga
     6361 ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg
     6421 cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc
     6481 cgaaaatgga tatacaagct cccgggagct ttttgcaaaa gcctaggcct ccaaaaaagc
     6541 ctcctcacta cttctggaat agctcagagg cagaggcggc ctcggcctct gcataaataa
     6601 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     6661 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac
     6721 ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca
     6781 tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca
     6841 cattccacag aattaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     6901 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     6961 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     7021 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     7081 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     7141 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     7201 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7261 taatgcgccg ctacagggcg cgtggggata ccccctagag ccccagctgg ttctttccgc
     7321 ctcagaagcc atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc
     7381 ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg
     7441 cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa
     7501 ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc
     7561 tgatcagcga gctctagcat ttaggtgaca ctatagaata gggccctcta gatgcatgct
     7621 cgagcggccg cttacttgtc atcgtcgtcc ttgtagtcca ggtcagtatc aaaccaggcc
     7681 agctgattgc tgtcacctgg aggcagccca tccatgaggt cctgggcatg ccccagatct
     7741 ggcagcccat caactggata gtcagcacca gggtgg
//
