LOCUS       sequence-105999-        8244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of tamoxifen-inducible Cre-ERT2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Kumar MS, Pester RE, Chen CY, Lane K, Chin C, Lu J, Kirsch DG, Golub
            TR, Jacks T
  TITLE     Dicer1 functions as a haploinsufficient tumor suppressor.
  JOURNAL   Genes Dev. 2009 Dec 1;23(23):2700-4. doi: 10.1101/gad.1848209
  PUBMED    19903759
REFERENCE   2  (bases 1 to 8244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2009-12-1- 1"; volume: "23"; issue:
            "23"; pages: "2700-4"
COMMENT     Sequence Label: MSCV CreERT2 puro
FEATURES             Location/Qualifiers
     source          1..8244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(607..626)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(754..776)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(970..989)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1089..1111
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1149..1167)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1235..1339
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1340..1408,1409..2200)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1558..1577)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2371..2959
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2860..2879
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3113..3130
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3247..3268
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3283..3288,3289..3306,3307..3313)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3321..3337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             3499..4013
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(4113..4712)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4196..4216)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4693..4712
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4733..5232)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4781..4800)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5174..5197
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(5243..6136)
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     CDS             complement(5249..6184)
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             complement(6197..7225)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVEDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             complement(7247..7663)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(7263..7279)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(7295..7317)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    7730..8071
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             join(8135..8244,1..407)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tacccgggcg acgcagtcta tcggaggact ggcgcgccga gtgaggggtt gtgggctctt
       61 ttattgagct cggggagcag aagcgcgcga acagaagcga gaagcgaact gattggttag
      121 ttcaaataag gcacagggtc atttcaggtc cttggggcac cctggaaaca tctgatggtt
      181 ctctagaaac tgctgagggc gggaccgcat ctggggacca tctgttcttg gccctgagcc
      241 ggggcaggaa ctgcttacca cagatatcct gtttggccca tattctgctg tctctctgtt
      301 cctaaccttg atctgaactt ctctattctc agttatgtat tttccatgcc ttgcaaaatg
      361 gcgttactta agctagcttg ccaaacctac aggtggggtc tttcattccc ccctttttct
      421 ggagactaaa taaaatcttt tattttatct atggctcgta ctctataggc ttcagctggt
      481 gatattgttg agtcaaaact agagcctgga ccactgatat cctgtcttta acaaattgga
      541 ctaatcgcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
      601 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
      661 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
      721 ccatctcctt gcatgcacca ttccttgcgc cgtcgtttta caacgtcgtg actgggaaaa
      781 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      841 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
      901 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
      961 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     1021 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     1081 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     1141 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     1201 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1261 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1321 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1381 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     1441 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     1501 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     1561 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     1621 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     1681 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1741 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1801 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1861 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1921 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1981 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     2041 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     2101 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     2161 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     2221 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     2281 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2341 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2401 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2461 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2521 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2581 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2641 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2701 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2761 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2821 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2881 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2941 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3001 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3061 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3121 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     3181 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     3241 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     3301 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacaattctc cccagctctt
     3361 tctgcgctgt ccataatgaa ctatttcagg agtattggag tatgtggatt caaaacagtg
     3421 gctaattgag tcaggattac ttaatattcg actcctaccc tcagaatgtt gttctccaac
     3481 ttcaagaaac tgttacccat gaaagacccc cgctgacggg tagtcaatca ctcagaggag
     3541 accctcccaa ggaacagcga gaccacaagt cggatgcaac tgcaagaggg tttattggat
     3601 acacgggtac ccgggcgacg cagtctatcg gaggactggc gcgccgagtg aggggttgtg
     3661 ggctctttta ttgagctcgg ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat
     3721 tggttagttc aaataaggca cagggtcatt tcaggtcctt ggggcaccct ggaaacatct
     3781 gatggttctc tagaaactgc tgagggcggg accgcatctg gggaccatct gttcttggcc
     3841 ctgagccggg gcaggaactg cttaccacag atatcctgtt tggcccatat tctgctgtct
     3901 ctctgttcct aaccttgatc tgaacttctc tattctcagt tatgtatttt ccatgccttg
     3961 caaaatggcg ttacttaagc tagcttgcca aacctacagg tggggtcttt cattcccccc
     4021 tttttctgga gactaaataa aatcttttat tttatcgatg catggggtcg tgcgctcctt
     4081 tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac
     4141 caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg
     4201 tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg
     4261 gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc
     4321 gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg
     4381 ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg
     4441 atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg
     4501 gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt
     4561 tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg
     4621 gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg
     4681 gcgaggcgca ccgtgggctt gtactcggtc atggtaagct tgggctgcag gtcgaaaggc
     4741 ccggagatga ggaagaggag aacagcgcgg cagacgtgcg cttttgaagc gtgcagaatg
     4801 ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct gagcccgccc ctgagcccgc
     4861 ccccggaccc accccttccc agcctctgag cccagaaagc gaaggagcaa agctgctatt
     4921 ggccgctgcc ccaaaggcct acccgcttcc attgctcagc ggtgctgtcc atctgcacga
     4981 gactagtgag acgtgctact tccatttgtc acgtcctgca cgacgcgagc tgcggggcgg
     5041 gggggaactt cctgactagg ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg
     5101 gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt
     5161 agcgccaagt gcccagcggg gctgctaaag cgcatgctcc agactgcctt gggaaaagcg
     5221 cctcccctac ccggtagaat tctcaagctg tggcagggaa accctctgcc tcccccgtga
     5281 tgtaatactt ttgcaaggaa tgcgatgaag tagagcccgc agtggccaag tggctttggt
     5341 ccgtctcctc cacggatgcc cctccacggc tagtgggcgc atgtaggcgg tgggcgtccg
     5401 ccgcctccag cagcaggtca tagaggggca ccacgttctt gcacttcatg ctgtacagat
     5461 gctccatgcc tttgttactc atgtgcctga tgtgggagag gatgaggagg agctgggcca
     5521 gccgctggtg ctgctgctgc agggtcaggc ctgccttggc catcaggtgg atcaaagtgt
     5581 ctgtgatctt gtccaggact cggtggatat ggtccttctc ttccagagac ttcagggtgc
     5641 tggacagaaa tgtgtacact ccagaattaa gcaaaataat agatttgagg cacacaaact
     5701 cctctccctg cagattcatc atgcggaacc gagatgatgt agccagcagc atgtcgaaga
     5761 tctccaccat gccctctaca cattttccct ggttcctgtc caagagcaag ttaggagcaa
     5821 acagtagctt cactgggtgc tccatggagc gccagacgag accaatcatc aggatctcta
     5881 gccaggcaca ttcaagaagg tggacctgat catggagggt caaatccaca aagcctggca
     5941 ccctcttcgc ccagttgatc atgtgaacca gctccctgtc tgccaggttg gtcagtaagc
     6001 ccatcatcga agcttcactg aagggtctgg taggatcata ctcggaatag agtatggggg
     6061 gctcagcatc caacaaggca ctgaccatct ggtcggccgt cagggacaag gccaggctgt
     6121 tcttcttaga gcgtttgatc atgagcgggc ttggccaaag gttggcagct ctcatgtctc
     6181 cagcagatgg ctcgagatcg ccatcttcca gcaggcgcac cattgcccct gtttcactat
     6241 ccaggttacg gatatagttc atgacaatat ttacattggt ccagccacca gcttgcatga
     6301 tctccggtat tgaaactcca gcgcgggcca tatctcgcgc ggctccgaca cgggcactgt
     6361 gtccagacca ggccaggtat ctctgaccag agtcatcctt agcgccgtaa atcaatcgat
     6421 gagttgcttc aaaaatccct tccagggcgc gagttgatag ctggctggtg gcagatggcg
     6481 cggcaacacc attttttctg acccggcaaa acaggtagtt attcggatca tcagctacac
     6541 cagagacgga aatccatcgc tcgaccagtt tagttacccc caggctaagt gccttctcta
     6601 cacctgcggt gctaaccagc gttttcgttc tgccaatatg gattaacatt ctcccaccgt
     6661 cagtacgtga gatatcttca accctgatcc tggcaatttc ggctatacgt aacagggtgt
     6721 tataagcaat ccccagaaat gccagattac gtatatcctg gcagcgatcg ctattttcca
     6781 tgagtgaacg aacctggtcg aaatcagtgc gttcgaacgc tagagcctgt tttgcacgtt
     6841 caccggcatc aacgttttct tttcggatcc gccgcataac cagtgaaaca gcattgctgt
     6901 cacttggtcg tggcagcccg gaccgacgat gaagcatgtt tagctggccc aaatgttgct
     6961 ggatagtttt tactgccaga ccgcgcgcct gaagatatag aagataatcg cgaacatctt
     7021 caggttctgc gggaaaccat ttccggttat tcaacttgca ccatgccgcc cacgaccggc
     7081 aaacggacag aagcattttc caggtatgct cagaaaacgc ctggcgatcc ctgaacatgt
     7141 ccatcaggtt cttgcgaacc tcatcactcg ttgcatcgac cggtaatgca ggcaaatttt
     7201 ggtgtacggt cagtaaattg gacatggtgt cgagagatct aattccggcg cctagagaag
     7261 gagtgagggc tggataaagg gaggatcgag gcggggtcga acgaggaggt tcaaggggga
     7321 gagacggggc ggatggagga agaggaggcg gaggcttagg gtgtacaaag ggcttgaccc
     7381 agggaggggg gtcaaaagcc aaggcttccc aggtcacgat gtaggggacc tggtctgggt
     7441 gtccatgcgg gccaggtgaa aagaccttga tcttaacctg ggtgatgagg tctcggttaa
     7501 aggtgccgtc tcgcggccat ccgacgttaa aggttggcca ttctgcagag cagaaggtaa
     7561 cccaacgtct cttcttgaca tctaccgact ggttgtgagc gatccgctcg acatctttcc
     7621 agtgacctaa ggtcaaactt aagggagtgg taacagtctg gccctaattt tcagacaaat
     7681 acagaaacac agtcagacag agacaacaca gaacgatgct gcagcgctgc agcagacaag
     7741 acgcgcggct tcggttccaa accgaaagca aaaattcaga cggaggcggg aactgtttta
     7801 ggttctcgtc tcctaccaga accacatatc ctgacggggt cggattccac atcgactccc
     7861 ttcctcaggt cgggccacaa aaacggcccc caaagtccct gggacgtctc ccagggttgc
     7921 ggccgggtgt tcagaactcg tcagttccac cacgggtccg ccagatacag agctagttag
     7981 ctaactagta cagacgcagg cgcaaacatt agatgccggc acaaacacgc acaaagacag
     8041 agacagacac gaaacgaccg ctggccagct tacctcccgg cgggggggtc ggtggtccct
     8101 gggcaggggt ctccaaatct cggtggaacc tccaaatgaa agacccccga ggtgggcagt
     8161 caatcaatct gaggagaccc tcccaaggat cagcgagtcc acgattcgga tgcaaacagc
     8221 aagaggcttt attgggaata cggg
//
