LOCUS       sequence-106064-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into 
            M3-M4 loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Wang Y, Xiao C, Indersmitten T, Freedman R, Leonard S, Lester HA
  TITLE     The duplicated alpha7 subunits assemble and form functional 
            nicotinic receptors with the full-length alpha7.
  JOURNAL   J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 
            10.1074/jbc.M114.582858. Epub 2014 Jul 23.
  PUBMED    25056953
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.582858"; journalName: "J Biol Chem"; date: 
            "2014-09-19- 19"; volume: "289"; issue: "38"; pages: "26451-63"
COMMENT     Sequence Label: pcDNA3.1-CHRNA7-mGFP
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
      121 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca
      181 ctggcggccg ctcgagcgga ctcaacatgc gctgctcgcc gggaggcgtc tggctggcgc
      241 tggccgcgtc gctcctgcac gtgtccctgc aaggcgagtt ccagaggaag ctttacaagg
      301 agctggtcaa gaactacaat cccctggaga ggcccgtggc caatgactcg caaccactca
      361 ccgtctactt ctccctgagc ctcctgcaga tcatggacgt ggatgagaag aaccaagttt
      421 taaccaccaa catttggctg caaatgtctt ggacagatca ctatttacag tggaatgtgt
      481 cagaatatcc aggggtgaag actgttcgtt tcccagatgg ccagatttgg aaaccagaca
      541 ttcttctcta taacagtgct gatgagcgct ttgacgccac attccacact aacgtgttgg
      601 tgaattcttc tgggcattgc cagtacctgc ctccaggcat attcaagagt tcctgctaca
      661 tcgatgtacg ctggtttccc tttgatgtgc agcactgcaa actgaagttt gggtcctggt
      721 cttacggagg ctggtccttg gatctgc
//
