LOCUS       sequence-106067-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human duplicated Alpha7 (CHRFAM7A) with YFP 
            fused into M3-M4 loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Wang Y, Xiao C, Indersmitten T, Freedman R, Leonard S, Lester HA
  TITLE     The duplicated alpha7 subunits assemble and form functional 
            nicotinic receptors with the full-length alpha7.
  JOURNAL   J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 
            10.1074/jbc.M114.582858. Epub 2014 Jul 23.
  PUBMED    25056953
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.582858"; journalName: "J Biol Chem"; date: 
            "2014-09-19- 19"; volume: "289"; issue: "38"; pages: "26451-63"
COMMENT     Sequence Label: pcDNA3.1-CHRFAM7A-YFP
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(384..635)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(426..447)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     775..794
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gggccctcta gactcgagcg gccgccactg tgctggatat ctgcagaatt gcccttcctt
       61 gcccatctgt gagttttcca catgtacaga accaggcgtg gttacgcaaa gtctttggac
      121 acggcctcca cgaagttggg agccgacatc aggatgccga tggtgcagat gatggtgaag
      181 accgagaagg ccatgaggca caggcggtcc accacacagg cggcgaactt ccactcgctg
      241 cagaccgcct cgctttcgtc ctggcagcgg aagcggttgg caatgtagcg gacctcctcc
      301 aggatcttgg ccaagtccgg gtccccctcg gggggttgcc cgccgtgcag gaggtgctca
      361 tcgtgcgtgg gggaggcgcc tgccttgtac agctcgtcca tgccgagagt gatcccggcg
      421 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
      481 gcggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      541 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
      601 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
      661 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      721 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      781 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
      841 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgca
//
