LOCUS       sequence-106083-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic circuit: OR gate, the sgRNA used for the NOT gate was 
            substituted with one designed to target the malT gene in the E. coli
            genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-OR-MalT-3NT
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(147..222)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(362..437)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      complement(534..561)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(577..648)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg gcggtcttaa
       61 gttttttggc tgaaaagcgg accaaaacga aaaaaggccc ccctttcggg aggcctcttt
      121 tctggaattt ggtaccgaga aaaaaagcac cgactcggtg ccactttttc aagttgataa
      181 cggactagcc ttattttaac ttgctatttc tagctctaaa acagcttttc attgagctgg
      241 cggctagcat aatacccgtg tgacccgttg gtgtaaaatg atttctggaa ttctaaagga
      301 ccaaaacgaa aaaagacgct tttcagcgtc ttattgttcg tctttggtac cgagaaaaaa
      361 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct
      421 atttctagct ctaaaacacg ggtcacacgg gtattatacc ttaacgatac ggtacgtttc
      481 gtatcatgtc aattggtaac gaatcagatt ccaccgtacg tcgcgtatat aaacgcagaa
      541 aggcccaccc gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg acaaacaaca
      601 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctgggggt
      661 atgggcccta gttaacgctg tgtacccgga caaacaccat taatcagcag gaaggtaaat
      721 tcttcaatat cctgttcaac ggtcagctgt tcggtcagca ggcgatacca acaaaaacca
      781 aaaatcatat ccagcagcag ttcacgattg gtatctttcg gcagttcacc attgctaatg
      841 gcatt
//
