LOCUS       sequence-106086-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic circuit: OR gate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-OR
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(150..225)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(365..440)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      complement(537..564)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(580..651)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta gtggacaaga ccggcggtct
       61 taagtttttt ggctgaaaag cggaccaaaa cgaaaaaagg cccccctttc gggaggcctc
      121 ttttctggaa tttggtaccg agaaaaaaag caccgactcg gtgccacttt ttcaagttga
      181 taacggacta gccttatttt aacttgctat ttctagctct aaaacccaca actagctggt
      241 attatgctag cataataccc gtgtgacccg ttggtgtaaa atgatttctg gaattctaaa
      301 ggaccaaaac gaaaaaagac gcttttcagc gtcttattgt tcgtctttgg taccgagaaa
      361 aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt attttaactt
      421 gctatttcta gctctaaaac acgggtcaca cgggtattat accttaacga tacggtacgt
      481 ttcgtatcat gtcaattggt aacgaatcag attccaccgt acgtcgcgta tataaacgca
      541 gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca ccgacaaaca
      601 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggg
      661 ggtatgggcc ctagttaacg ctgtgtaccc ggacaaacac cattaatcag caggaaggta
      721 aattcttcaa tatcctgttc aacggtcagc tgttcggtca gcaggcgata ccaacaaaaa
      781 ccaaaaatca tatccagcag cagttcacga ttggtatctt tcggcagttc accattgcta
      841 atggcattt
//
