LOCUS       sequence-106095-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human duplicated Alpha7-2 bp deletion 
            (CHRFAM7A-∆2bp) with mEGFP fused into M3-M4 loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Wang Y, Xiao C, Indersmitten T, Freedman R, Leonard S, Lester HA
  TITLE     The duplicated alpha7 subunits assemble and form functional 
            nicotinic receptors with the full-length alpha7.
  JOURNAL   J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 
            10.1074/jbc.M114.582858. Epub 2014 Jul 23.
  PUBMED    25056953
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.582858"; journalName: "J Biol Chem"; date: 
            "2014-09-19- 19"; volume: "289"; issue: "38"; pages: "26451-63"
COMMENT     Sequence Label: pcDNA3.1-CHRFAM7ADelta2bp-mGFP
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
      121 gagctcggat ccactagtcc agtgtggtgg aattgccctt ctcggtgccc cttgccattt
      181 tccagccgcg ctcccacgag ggtcacggcg gcggggagag gtggagccgc gagagctcgg
      241 ccgggggccc cgcctggtgg tcgcggccat gacagcggct cgggacaggc tccttttccg
      301 cgcccctccc gccggaggtg aggggaagat gtccatgtca gggttcaagg ccaaaccgaa
      361 gttactggcc tctatcttcc aggagaacca ggagccacag ccgcggctca cgccccaccg
      421 caacattaag attacaagtg gacacctgag tcagcaggac ctggaatccc agatgagaga
      481 gcttatctac acgactcaga tcttgttgtc acccccatta ttgacaatcc aaaggtgcag
      541 aaagcactct gacaaataat gaaacaacca ccatcggtta aatttgatgc aaaaatattg
      601 catctaccag cattttcagt tccaattgct aatccagcat ttgtggatag ctgcaaactg
      661 tgatattgct gatgagcgct ttgacgccac attccacact aacgtgttgg tgaattcttc
      721 tgggcattgc cagtacctgc ctccaggcat attcaagagt tcctg
//
