LOCUS       sequence-106101-         404 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK 
            RNA polymerase III promoter for shRNA expression. Compatible with 
            MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR 7SK L5-L4
FEATURES             Location/Qualifiers
     source          1..404
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..121
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cgcggccgcc ctgcagctgg atggcaaata atgattttat tttgactgat agtgacctgt
       61 tcgttgcaac aaattgataa gcaatgcttt cttataatgc caactttgta tagaaaagtt
      121 gggtgcgaat tcaaaaaaga gctctagatc tgtgaagacc gaggtaccca ggcggcgcac
      181 aagctatata aacctgaagg aaatctcaac tttacactta ggtcaagtta cttatcgtac
      241 tagagcttca gcaggaaatt taactaaaat ctaatttaac cagcatagca aatatcattt
      301 attcccaaaa tgctaaagtt tgagataaac ggacttgatt tccggctgtt ttgacactat
      361 ccagaatgcc ttgcagatgg gtggggcatg ctaaatactg cagc
//
