LOCUS       sequence-106107-         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter for shRNA expression. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR U6 L1-L4
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..121
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(359..599)
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     complement(409..428)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(579..599)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 cgcggccgcc ctgcagctgg atggcaaata atgattttat tttgactgat agtgacctgt
       61 tcgttgcaac aaattgataa gcaatgcttt cttataatgc caactttgta tagaaaagtt
      121 gggtgcgaat tcaaaaaaga gctcggactc gggcgccggc ctggagctgg gcatcgcgtc
      181 ctccccgacg cctggctcca ccgcgtcgac gggcggcaag gcggacgacc ccagctggtg
      241 caagacgccc agtggccaca tcaagcggcc catgaacgcc tttatggtgt ggtcgcagat
      301 cgagcggcgc aagatcatgg agcagtcgcc cgacatgcac aacgccgaga tctgtttcgt
      361 cctttccaca agatatataa agccaagaaa tcgaaatact ttcaagttac ggtaagcata
      421 tgatagtcca ttttaaaaca taattttaaa actgcaaact acccaagaaa ttattacttt
      481 ctacgtcacg tattttgtac taatatcttt gtgtttacag tcaaattaat tccaattatc
      541 tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa taggccctcg
      601 gtgaaggggg cggccgctcg aggctagtct cggatcgata gagcctgc
//
