LOCUS       sequence-106131-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast 
            resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena 
            C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
  TITLE     Genome-scale transcriptional activation by an engineered CRISPR-Cas9
            complex.
  JOURNAL   Nature. 2014 Dec 10. doi: 10.1038/nature14136.
  PUBMED    25494202
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Dec 10. doi: 10.1038/nature14136."
COMMENT     Sequence Label: lenti dCAS-VP64_Blast
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..73
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ggtgtcgtga cgtacggcca ccatgaaaag gccggcggcc acgaaaaagg ccggccaggc
       61 aaaaaagaaa aaggacaaga agtacagcat cggcctggcc atcggcacca actctgtggg
      121 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
      181 caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga
      241 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
      301 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
      361 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
      421 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
      481 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc
      541 cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga
      601 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
      661 ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag
      721 caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct
      781 gttcggcaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
      841 cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa
      901 cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc
      961 cgacgcc
//
