LOCUS       sequence-106134-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast 
            resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena 
            C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
  TITLE     Genome-scale transcriptional activation by an engineered CRISPR-Cas9
            complex.
  JOURNAL   Nature. 2014 Dec 10. doi: 10.1038/nature14136.
  PUBMED    25494202
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Dec 10. doi: 10.1038/nature14136."
COMMENT     Sequence Label: lenti dCAS-VP64_Blast
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..65)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(87..236)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(261..281)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggcttggcca ttgggccagg attctcctcg acgtcaccgc atgttagcag acttcctctg
       61 ccctctccac tgcctgtaca gttaatcagc atgtccaggt cgaaatcatc aagggcgtca
      121 ctgccgagca tgtcgaggtc aaagtcatca agggcatccg aaccaagcat gtcaaggtca
      181 aaatcatcga gggcgtcact tcccagcata tccagatcaa aatcgtccaa tgcgtcagcc
      241 cgtccggatc cagcggccgc caccttcctc tttttcttag gtccgctacc tcctcctccg
      301 cttcctcctc ctccgcttcc acctcctcca gcgctgtcgc ctcccagctg agacaggtcg
      361 atccgtgtct cgtacaggcc ggtgatgctc tggtggatca gggtggcgtc cagcacctct
      421 ttggtgctgg tgtacctctt ccggtcgatg gtggtgtcaa agtacttgaa ggcggcaggg
      481 gctcccagat tggtcagggt aaacaggtgg atgatattct cggcctgctc tctgatgggc
      541 ttatcccggt gcttgttgta ggcggacagc actttgtcca gattagcgtc ggccaggatc
      601 actctcttgg agaactcgct gatctgctcg atgatctcgt ccaggtagtg cttgtgctgt
      661 tccacaaaca gctgtttctg ctcattatcc tcgggggagc ccttcagctt ctcatagtgg
      721 ctggccaggt acaggaagtt cacatatttg gagggcaggg ccagttcgtt tcccttctgc
      781 agttcgccgg cagaggccag cattctcttc cggccgtttt ccagctcgaa cagggagtac
      841 ttaggcagct tgatgatcag gtcctttttc acttctttgt agcccttggc ttccagaaag
      901 tcgatgggat tcttctcgaa gctgcttctt tccatgatgg tgatccccag cagctctttc
      961 acactcttca gtttcttgga cttgcccttt t
//
