LOCUS       sequence-106146-         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  conditional gene knockdowns in Trypanosoma brucei.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Brandenburg J, Schimanski B, Nogoceke E, Nguyen TN, Padovan JC, 
            Chait BT, Cross GA, Gunzl A
  TITLE     Multifunctional class I transcription in Trypanosoma brucei depends 
            on a novel protein complex.
  JOURNAL   EMBO J. 2007 Nov 28;26(23):4856-66. Epub 2007 Nov 1.
  PUBMED    17972917
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2007-11-28"; volume: "26(23)"; pages: "4856-66. Epub 2007 Nov
            1"
COMMENT     Sequence Label: pT7-stl
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(231..249)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        260..278
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    283..301
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    302..320
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(461..479)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(561..579)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gggagaaccc gagccggtcg gtccagaact cgaccgctcc ggcgacgtcg cgcgcggtga
       61 gcaccggaac ggcactggtc aacttggcca tggtgatagc ttattttatg gcagcaacga
      121 gaccttacgt aaagcaaaac taaataagag cggagactgc aatgcagagt aaaaaaaaaa
      181 aaaacatgaa tttcagaaga ccttgctgtg cccccggtac gggagatctc cctatagtga
      241 gtcgtattaa tcaggtacct aatacgactc actataggga tctccctatc agtgatagag
      301 atccctatca gtgatagaga agcttggaat tcctttgtgt tacattcttg aatgtcgctc
      361 gcagtgacat tagcattccg gtactgttgg taaaatggaa gacgccaaaa acataaagaa
      421 aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac tgcataaggc
      481 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt
      541 gaacatcacg tacgacgcgt ccgctgagca ataactagca taaccccttg gggcctctaa
      601 acgggtcttg a
//
