LOCUS       sequence-106149-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Small IncP-based broad host range replicating vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Puri AW, Owen S, Chu F, Chavkin T, Beck DA, Kalyuzhnaya MG, Lidstrom
            ME
  TITLE     Genetic tools for the industrially promising methanotroph 
            Methylomicrobium buryatense.
  JOURNAL   Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
  PUBMED    25548049
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14."
COMMENT     Sequence Label: pAWP78
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(329..917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(409..428)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
       61 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
      121 ttacgctgac ttgacgggac ggcggctttg ttgaataaat cgaacttttg ctgagttgaa
      181 ggatcagatc acgcatcttc ccgacaacag ctcactcaaa ggcggtaata cggttatcca
      241 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      301 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      361 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      421 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      481 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      541 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      601 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      661 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      721 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      781 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      841 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      901 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
      961 acgaaaactc acgttaaggg attttggtca tgattcgcaa cctagtgaat gagcggtacg
     1021 aactttcgca gagtgcgaat agcgaccgag caatcgagat acgtaccgtt agattcacgc
     1081 tccagacagc ac
//
