LOCUS       sequence-106161-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic circuit: AND gate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-AND
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(137..212)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      complement(309..336)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(352..423)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 accgccgatc ctgtccaccg cattactgca aggtagtgga caagaccggc ggtcttaagt
       61 tttttggctg aaggaccaaa acgaaaaaag acgcttttca gcgtcttatt gttcgtcttt
      121 ggtaccgaga aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc
      181 ttattttaac ttgctatttc tagctctaaa acccacaact agctggtatt ataccttaac
      241 gatacggtac gtttcgtatc atgtcaattg gtaacgaatc agattccacc gtacgtcgcg
      301 tatataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt
      361 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
      421 ttgatgcctg ggggtatggg ccctagttaa cgctgtgtac ccggacaaac accattaatc
      481 agcaggaagg taaattcttc aatatcctgt tcaacggtca gctgttcggt cagcaggcga
      541 taccaacaaa aaccaaaaat catatccagc agcagttcac gattggtatc tttcggcagt
      601 tcaccattgc taatggcatt ttcaaccagt tttttcggca tctcacgacg acgttccata
      661 aactgatctt tcagctgggt cagggttgca gggtccagct gtgcttctgc aataacacaa
      721 cgaaatgctt caccacaaat ggtttcacgc caaactttcc acagattacg cagcagaa
//
