LOCUS       sequence-106163-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human BRAT1 with an N-terminal HA tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   So EY, Ouchi T
  TITLE     BRAT1 deficiency causes increased glucose metabolism and 
            mitochondrial malfunction.
  JOURNAL   BMC Cancer. 2014 Jul 29;14:548. doi: 10.1186/1471-2407-14-548.
  PUBMED    25070371
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Cancer."; date: "2014-07-29"; volume: "14:548. doi"; pages: " 
            10.1186/1471-2407-14-548"
COMMENT     Sequence Label: pcDNA3-HA-BRAT1
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttggtac
       61 cgagctcgga tcgatgtacc catacgatgt tccagattac gctggatccg aattcggcac
      121 gagggttgcg gcggaggacg caaccgagcg ggcctgcggc ctcaccatgg acccagaatg
      181 cgcccagctg ctcccggctc tctgtgctgt tctggtagat cccgggcagc cggtggcaga
      241 tgacacctgt ttggagaagc tcctggactg gtttaaaacg gtcactgaag gagagtccag
      301 tgtcgtgctg ctgcaggagc acccctgcct ggtggagctg ctgtcccatg tgctgaaagt
      361 ccaggacctg agttctgggg tcctctcctt ctcactgcgc ctggcaggaa ccttcgcagc
      421 ccaggaaaac tgcttccagt atcttcagca gggggagtta ctaccagggc tctttgggga
      481 gccaggaccc ctcggccgag caacctgggc cgtccccacc gtgcgcagcg gctggatcca
      541 gggcctgcgc tccctggcac agcaccccag cgccctgcgc ttcctggccg accatggtgc
      601 ggtcgacacc atcttctccc tgcagggaga ctccagcctg tttgtggcct cggcggccag
      661 tcagctcctg gtgcacgtcc tggctttgtc catgcgaggt ggagccga
//
