LOCUS       sequence-106180-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Yang J, Sanderson NS, Wawrowsky K, Puntel M, Castro MG, Lowenstein 
            PR
  TITLE     Kupfer-type immunological synapse characteristics do not predict 
            anti-brain tumor cytolytic T-cell function in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Mar 9. 107(10):4716-21.
  PUBMED    20133734
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Mar 9. 107(10):4716-21."
COMMENT     Sequence Label: pCI-neo-cOVA
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          177..309
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     292..311
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        354..372
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             788..811
                     /codon_start=1
                     /product="antigenic CD8+ T cell epitope from chicken 
                     ovalbumin"
                     /label=OVA peptide
                     /translation="SIINFEKL"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt
       61 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
      121 ctgcagtgac tctcttaagg tagccttgca gaagttggtc gtgaggcact gggcaggtaa
      181 gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga
      241 gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc
      301 tctccacagg tgtccactcc cagttcaatt acagctctta aggctagagt acttaatacg
      361 actcactata ggctagcctc gagaattcgt cgacatgggc tccatcggcg cagcaagcat
      421 ggaattttgt tttgatgtat tcaaggagct catcaattcc tgggtagaaa gtcagacaaa
      481 tggaattatc agaaatgtcc ttcagccaag ctccgtggat tctcaaactg caatggttct
      541 ggttaatgcc attgtcttca aaggactgtg ggagaaaaca tttaaggatg aagacacaca
      601 agcaatgcct ttcagagtga ctgagcaaga aagcaaacct gtgcagatga tgtaccagat
      661 tggtttattt agagtggcat caatggcttc tgagaaaatg aagatcctgg agcttccatt
      721 tgccagtggg acaatgagca tgttggtgct gttgcctgat gaagtctcag gccttgagca
      781 gcttgagagt ataatcaact ttgaaaaact gactgaatgg accagttcta atgttatgga
      841 agagaggaag atcaaagtgt acttacctcg catgaagatg gaggaaaaat acaacctcac
      901 atctgtctta atggctatgg gcattactga cgtgtttagc tcttcagcca atctgtctgg
      961 catctcctca gcagagagcc tgaagatatc tcaagctgtc catgcagcac atgcagaaat
     1021 caatgaagca ggcagagag
//
