LOCUS       sequence-106182-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Yang J, Sanderson NS, Wawrowsky K, Puntel M, Castro MG, Lowenstein 
            PR
  TITLE     Kupfer-type immunological synapse characteristics do not predict 
            anti-brain tumor cytolytic T-cell function in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Mar 9. 107(10):4716-21.
  PUBMED    20133734
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Mar 9. 107(10):4716-21."
COMMENT     Sequence Label: pCI-neo-cOVA
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    258..379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(295..314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     349..368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(832..855)
                     /codon_start=1
                     /product="antigenic CD8+ T cell epitope from chicken 
                     ovalbumin"
                     /label=OVA peptide
                     /translation="SIINFEKL"
ORIGIN
        1 ctggcagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
       61 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
      121 ggcctcttcg ctattacgcc agcccggatc gatccttatc ggattttacc acatttgtag
      181 aggttttact tgctttaaaa aacctcccac atctccccct gaacctgaaa cataaaatga
      241 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      301 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      361 aactcatcaa tgtatcttat catgtctgct cgaagcatta accctcacta aagggaagcg
      421 gccgcccggg tcgactctag aggtaccacg cgtgaattcg tcgacttaag gggaaacaca
      481 tctgccaaag aagagaacgg cgttggttgc gatgtgcttg atacagaaga ggaatggatg
      541 gtcagcccta aattcttcag agacgcttgc agcatccact ccagcctctg ctgaccctac
      601 cacctctctg cctgcttcat tgatttctgc atgtgctgca tggacagctt gagatatctt
      661 caggctctct gctgaggaga tgccagacag attggctgaa gagctaaaca cgtcagtaat
      721 gcccatagcc attaagacag atgtgaggtt gtatttttcc tccatcttca tgcgaggtaa
      781 gtacactttg atcttcctct cttccataac attagaactg gtccattcag tcagtttttc
      841 aaagttgatt atactctcaa gctgctcaag gcctgagact tcatcaggca acagcaccaa
      901 catgctcatt gtcccactgg caaatggaag ctccaggatc ttcattttct cagaagccat
      961 tgatgccact ctaaataaac caatctggta catcatctgc acaggttt
//
