LOCUS       sequence-106186-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Alexeyev MF
  TITLE     The pKNOCK series of broad-host-range mobilizable suicide vectors 
            for gene knockout and targeted DNA insertion into the chromosome of 
            gram-negative bacteria.
  JOURNAL   Biotechniques. 1999 May;26(5):824-6, 828.
  PUBMED    10337469
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "1999-05"; volume: "26(5)"; pages: "824-6, 
            828"
COMMENT     Sequence Label: pKNOCK-Cm
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     71..87
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     71..87
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(121..137)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(122..138)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        271..373
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(440..459)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agcattttga gtgacacagg aacacttaac ggctgacatg ggaattcccc tccaccgcgg
       61 tggcggccgc tctagaacta gtggatcccc cgggctgcag gaattcgata tcaagcttat
      121 cgataccgtc gacctcgagg gggggcccgg taccgaggac gcgtccgaat aaatacctgt
      181 gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata ccgggaagcc
      241 ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt ccaactttca
      301 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc
      361 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
      421 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
      481 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
      541 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc
      601 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
      661 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
      721 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
      781 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
      841 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
      901 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
      961 tgatggcttc catg
//
