LOCUS       sequence-106193-        9627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3.2 kb marine armor plate enhancer driving GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   O'Brown NM, Summers BR, Jones FC, Brady SD, Kingsley DM
  TITLE     A recurrent regulatory change underlying altered expression and Wnt 
            response of the stickleback armor plates gene EDA.
  JOURNAL   Elife. 2015 Jan 28;4:e05290. doi: 10.7554/eLife.05290.
  PUBMED    25629660
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-01-28"; volume: "4:e05290. doi"; pages: " 
            10.7554/eLife.05290"
COMMENT     Sequence Label: p3.2mar-GFP
FEATURES             Location/Qualifiers
     source          1..9627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(561..572,573..735)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      5859..5868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5865..5867,5868..5870,5871..6584)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5865..5867,5868..5870,5871..6581)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5910..5931)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6171..6190)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6518..6539
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6600..6734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6624..6643)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6678..6697
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(7208..7395,7396..7407)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7443..7463)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7443..7461)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7482..7498)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7482..7498)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7495..7517)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7506..7522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7530..7536,7537..7554,7555..7560))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7575..7596
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7713..7730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7884..8472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7964..7983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8643..9434,9435..9503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9266..9285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9504..9608)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggccac aatgacggct actacatggt
      361 gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaagg ctcttggata
      421 cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct
      481 gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag
      541 gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa
      601 agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct
      661 tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa
      721 aagtaaaagc aagaaagaaa actagagatt cttgtttaag cttttaatct caaaaaacat
      781 taaatgaaat gcatacaagg ttttatcctg ctttagaact gtttgtattt aattatcaaa
      841 ctataagaca gacaatctaa tgccagtaca cgctactcaa agttgtaaaa cctcagattt
      901 aacttcagta gaagctgatt ctcaaaattg ttagtgtcaa gcctagctct tttggggctg
      961 aaaagcaatc ctgcagtgct gaaaagcctc tcacaggcag ccgatgcggg aagaggtgta
     1021 ttagtcttga tagagaggct gcaaatagca ggaaacgtga gcagagactc cctggtgtct
     1081 gaaacacagg ccagataggc cacatggtgt gggagagcag tgatcgttct ttcaaactat
     1141 ttcactgttt gttttgtttt agtcatcatc atcgtctcct ttgtttcccc aacagtgttg
     1201 aaggccagaa attgatcatg tctctctccc ttattgtttc aggggttccc gataagacaa
     1261 aaacaaaaga attccaggta gagcatgtca gctattgaag taaagtctta ccacagacac
     1321 aagcgctgat aaaacagcgg ctgctttgtt taaatgtgta tcatgtgttg ctcaccaggg
     1381 atcttctttc tttattttct ttcagcctgc agtggttcat ttgcagggtc aggagacgac
     1441 catccaagtg agagaaggtg gggcttccgt ccgctcggtc tcttgtagtt gcgccgctgt
     1501 ctttgacgca aagtcattaa cgtgtcaaca ccctttgtca cagaaccggg cctgcaactg
     1561 ttaccgctac acacggactt acaccacaac gaatatataa cctgctaacc atatttactt
     1621 tactagggtt gtgtatgatt ttggttaagc ggaagtaatc ttctagctat tacacgatta
     1681 gttcagtagg cttgtatttt aaatcagacg tgtcagactt ggccagagct gaaccctcga
     1741 atgttagcag gttgaagtca tcctgcatca tttgtgtgtg tgtgtgtgtg agaatcatga
     1801 gatggtttat atattccgcc agtgctgagc tctggttgta gttaaaccct gctgagagct
     1861 gttttatgtt gactgtgcgc ctcttgtttg tttgttgttg catgcttaga tctttctgaa
     1921 ggcatcctca ggaactggaa gatggtgtcc atccatcacc gggtgtttaa aatgcactcc
     1981 cgctctggag agctagaggt gctgctggac ggtgtctact tcatctatag tcaggtagaa
     2041 gtgagtacgg tccttgacgt aactcttatg atttacagtt cggagtgaaa agaactggca
     2101 gcttctctgt agctaagcgt aaaaacctgt tccattgatc tacccatcaa tgtttgactt
     2161 ttattatgag aatattttca ctctccctag gtggacgtct ataactcaaa cccgtgttcg
     2221 ctactccgaa attccagctg ttctagtttt taaataatct ctttaatatc gtgtgtggat
     2281 ctcttttgac tacccctctt agtcggctgt gaattgtatg tcctcgccag gtgtactacc
     2341 tcaacttcac cgacattgcc agctacgagg tgatggtgga ttccaacccg ttcctccgct
     2401 gcacctgcag catcgagacg ggccagcgca agttcaacac gtgctacacg gccggggtca
     2461 gcctgctccg tgccggccag aggatctcca tacgcatagt gtatgaggac acgctcatca
     2521 gcatgaccaa ccacaccacc ttcctgggaa gcgtcaggct tggagaggct ccatctgctg
     2581 gacaaaactg ataactacac aaccaccggg cctctacgtg aaatgccccc cacccctcct
     2641 tcccacactc tgttcagagg aaacttaaat cctcccagta attgacattg aggaaccccc
     2701 cccccccccc acttggactt ggggacttat gtcaccttca caggtctgca ttaagaagaa
     2761 gtgtatagac cgccccactt tgccctcacg ttgactctgt gcaaagagat cagccgtgat
     2821 gagcctaaaa aacaaaaaaa agggtttcta tttctagtca ccatctggtg tcaacacaga
     2881 cgacatctct ctttataacc agaaaggaca ctgcgctgac atatacttaa tatttacata
     2941 ttgtcaagtg ctgctgaatg ataatctcgt atcgccaaaa aactccagct attccaaatg
     3001 ctctatttgc agttttttat agttggtcat tttaattcat tgaattatat taacaggtat
     3061 aagtaggtgg tttaacatgc gcttattagc accatatgcc actgggttat tcaaaaaaag
     3121 gttcaaagca agtataaaga tatctatgca aggctgagaa aagaaacatt aaaactggag
     3181 ttataaggta ttcacagacc aacagcacta tatattttgc acatatttgc actacagcat
     3241 ccctgcagaa tatcagagat atgttttgtt acagtgctat tgtttggtca ccgtatgaat
     3301 gaatccatgt ggaaaaatga tagttgttta tacgtctctg tacatattgt atataaaaag
     3361 ggatatactt ttgtaaatgg agttgattga tggattaaaa tgtttaattt tctgtctaaa
     3421 tgcatttgtc tgtcttgtgt ttgtgtagct atggtattga tttaaagggc taaacgtgtc
     3481 tcctattttg gacactgctg gcacgggttt tgacaagtga tgttctctgt ggcctttggc
     3541 tccattaaaa tgttgctttt actttgcaag ttttacaaca cacttcaaat ttggctgtaa
     3601 tcttcgtagt atctaaatta gttccatctt gagagtcaga gagaagatgg ttcctcattt
     3661 tgaaacttta catttaggtt tccgggggtt aaccagagtg tgacctcacc catttgagta
     3721 acttcaggac atgcagtctg cgtcttcctt ctcacgatgc tttacagcgc cttattgtca
     3781 ctagcagcat ttatgttttg tcttagtgtt ttcctggtgc acagagccag tgttttagaa
     3841 aatgatttgc gcaaactcca aggggacata attctacaat tcaatcagcc acgttctgag
     3901 ggagtcaatg ggaaaatggc caagatgtct gaaagaaggg aggtatgtca ctgattatgg
     3961 aggatgtagt catcttggac attgtactac tgagatttat gccattagtg agtgttcaga
     4021 tagcgctgca ataacacatt ttgtcctaat gctctgtttt ctctaaggac ataaagccaa
     4081 gtactttgcc aaacagtctg agttctctga aggtttcttc caggagagtg aaacggcaac
     4141 agggcagctg cagaggtgaa ctaaaaatgt gtccattagt tacggtctaa tcttgtagtg
     4201 agaccataaa cactatatac taagttaatt gaattaaagt attatgttta tttcctcctc
     4261 ctgccagctc caacaggcca gataggcccc ttacgtacgc tagcgggccc gatatcaccg
     4321 ggggatcctt caggggtgtc gcttggttat ttccaaaaat caaattaatt ttattaaact
     4381 attagaacga gcatgttttg tctatatgct acagaagata aaaaataata ggagttaaca
     4441 gttataaaac aacacacttt gtttctattg attgttgacc acactggggt ctcattaagt
     4501 tagattaaag acacactaac tgggtcaaaa gcagcagatt gatttcatag caccagggta
     4561 aactttctaa cacttttacg gcaatcatat acattaaaat taaatacaga ccacgactga
     4621 acaaggagga tgatctccaa tattaaacaa agagacttgt gcctatttct ctgagggtaa
     4681 acatgacctc tcaagttagc aagttgtttt taacactaca aaaatagtta agactgcaat
     4741 cccagaataa agtattggtt ttaaccaatc aatatagtac agtaaacatc catttgtttt
     4801 gttgaaacgt taaacaaatc tgaccaaagc tattagctta tataaaacag gtttgccttc
     4861 tatgtagctg aaaacaccac aggcccgatt ttgctactgt gtaaaacatt tcagcaagat
     4921 ttttttattg catttttttt tactgaatcg ttcaaacatt ttatcatttt agtttgttca
     4981 ttcattgcaa ctggaaaaac aacacatcac acaaccgcac atatttcagc aataagtaca
     5041 ataaaacact caaataaaaa aaacatttta aatctctttg tatttttgac cgctgtttcg
     5101 cgtaatttca cggtaaaact ctggaaatct ccactacatt cctctcagcg gctcctctca
     5161 atgacagctg aagaagtgac gcggctgcct gctgtgtttt gattggtcga attcactgga
     5221 ggcttccaga acagtgtaga gtctgaacgg gtgcgcgctc tgctgtattt aaagggcgaa
     5281 agagagaccg cagagaaact caaccgaaga gaagcgactt gacaaagaag aaaagagcag
     5341 cctgacagga ctttttcccc gacgaggtgt ttattcgctc tatttaagaa tctactgtaa
     5401 ggtaagtctc aatatattgt actctattgg ctaatcagaa ttatatagag attatatgta
     5461 cttaatgtca aaaaatcaac tttgtatatg taatcttttt acatgtggac tgcctatgtt
     5521 catcttattt taggtctact agaaaattat atttcccgtt ttcacaataa ggatttttaa
     5581 aaaaagcaat gaacagacgg gcatttactt tatgttgctg acattatttt atatgagcat
     5641 aataaccata aatactagca aatgtcctaa atgaatttgt gttaatgttg tctacaaaag
     5701 aaaattagcg ttttacttgt acaactaata ataacttggt tattaagaga atttcacttg
     5761 ttgactagaa aaatcctttc ataatgaaac aattgcacca taaattgtat aaatataaaa
     5821 ttaattctaa ttgttttttt ttcctgcagt cgacggtcgc caccatggtg agcaagggcg
     5881 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     5941 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     6001 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     6061 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     6121 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     6181 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     6241 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     6301 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     6361 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     6421 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     6481 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     6541 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcgac atcgatgatg
     6601 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     6661 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     6721 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     6781 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     6841 ctctagatct tgaatgaatc tttatttaca ccacttaaag aaaagaattc gcgaagatac
     6901 ggccacgggt gctcttgatc ctgtggctga ttttggactg tgctgctcgc agctgctgat
     6961 gaatcacata cttcctccat tttcttccac tgattgactg ttataatttc cctaatttcc
     7021 aggtcaaggt gctgtgcatt gtggtaatag atgtgacatg acgtcacttc caaaggacca
     7081 atgaacatgt ctgaccaatt tcatataatg tgaaaacgat tttcataggc agaataaata
     7141 acatttaaat taaactgggc atcagcgcaa ttcaattggt ttggtaatag caagggaaaa
     7201 tagaatgaag tgatctccaa aaaataagta ctttttgact gtaaataaaa ttgtaaggag
     7261 taaaaagtac ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt attgattttt
     7321 aattgtactc aagtaaagta aaaatcccca aaaataatac ttaagtacag taatcaagta
     7381 aaattactca agtactttac acctctgcgg ccgccaccgc ggtggagctc cagcttttgt
     7441 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     7501 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7561 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7621 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7681 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7741 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7801 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8281 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     8341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8521 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8581 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8641 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8701 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8761 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8821 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8881 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8941 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9001 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9061 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9121 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9181 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9241 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9301 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9361 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9421 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9481 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9541 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9601 gttccgcgca catttccccg aaaagtg
//
