LOCUS       sequence-106202-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A single vector AAV-Cas9 system containing Cas9 from Staphylococcus 
            aureus (SaCas9) and its sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B,
            Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F
  TITLE     In vivo genome editing using Staphylococcus aureus Cas9.
  JOURNAL   Nature. 2015 Apr 1. doi: 10.1038/nature14299.
  PUBMED    25830891
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Apr 1. doi: 10.1038/nature14299."
COMMENT     Sequence Label: 
            pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     131..150
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        229..304
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     polyA_signal    316..523
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     512..529
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(557..598)
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             complement(599..625)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(632..679)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
       61 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
      121 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
      181 atatatcttg tggaaaggac gaaacaccgg agaccacggc aggtctcagt tttagtactc
      241 tggaaacaga atctactaaa acaaggcaaa atgccgtgtt tatctcgtca acttgttggc
      301 gagatttttg gtacctcccc agcatgcctg ctattctctt cccaatcctc ccccttgctg
      361 tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt
      421 tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg
      481 ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcga
      541 gctctaggaa ttcttacttt cccatcaacc tgggtccaag ctcgttggcg aagccgctag
      601 cgtaatctgg aacatcgtat gggtaggatc cctttttctt ttttgcctgg ccggcctttt
      661 tcgtggccgc cggccttttg ccctttttga tgatctgagg gtgcttctta gatttcactt
      721 catacaggtt gcccagaatg tctgtgctgt acttcttaat gctctgggtc ttggaggcga
      781 ttgtcttaat gatcctgggg ggcctcttgt cgttcatgtt ttccaggtac tcgcggtagg
      841 tgatgtcgat catgttcact tcgatccggt tcagcaggtc gttgttcacg ccgatcactc
      901 tatacagctc gccgttgatc ttgatcagat cgttgttgta gaaggaggcg ataaactcgg
      961 cctggttgct gatcttcttc agcttcttag cttcctca
//
