LOCUS       sequence-106204-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A single vector AAV-Cas9 system containing Cas9 from Staphylococcus 
            aureus (SaCas9) and its sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B,
            Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F
  TITLE     In vivo genome editing using Staphylococcus aureus Cas9.
  JOURNAL   Nature. 2015 Apr 1. doi: 10.1038/nature14299.
  PUBMED    25830891
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Apr 1. doi: 10.1038/nature14299."
COMMENT     Sequence Label: 
            pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttcttggtct tgctgattct gcccttgccc ttggccagat tcaggatgtg cttcttgaag
       61 gtttcgtagc tgatcttgct gtcgctgctg ctcaggtact ggaatggggt ccggttgccc
      121 ttcttgctgt tttcttcctg cttcacgagc accttgttgt tgaagctgtt gtcgaaggac
      181 acgcttctgg ggatgatgtg gtccacctca tagttgaagg ggttgttcag cagatcttcc
      241 agagggatgg cttccaggct gtacaggcac ttgccttcct gcatgtcgtg cagcttgatc
      301 ttctcgatca ggtacttggc gttctctttg ccggtggtcc ggatgatttc ctcgatccgc
      361 tcgttggtct gccggttccg cttctgcatc tcgttgatca ttttctgggc gtccttggag
      421 ttcttctcgc gggccagctc gataatgatg tcgttgggca ggccgtactt cttgatgatg
      481 gcgttgatca ctttgatgct ctggatgaag cttctcttca cgacggggct caggatgaag
      541 tcgtccacca gggtggtggg gatctctttc tgctgggaca ggtccacctt cttgggcacc
      601 agcttcagcc ggttgaagat agcgatctgg ttgtcgttgg tgtgccacag ctcgtccagg
      661 atcaggttga tggccttcag gctcaggttg tgggtgccgg tatagccctt cagattagag
      721 atctgctcga tctcttcctg ggtcagctcg gagttcagat tggtcagttc ttcctggatg
      781 tcctcgctgc tctggtagat ggtcaggatc ttggcaatct gatccagcag ctcggcgttc
      841 tcaataatct ctttccgggc ggtaatgtcc ttgatgtcgt ggtacacctt caggttggtg
      901 aactcgggct tgccggtgct ggtcactctg tagcccttaa tatcctcttc gttcacgagg
      961 atttctttgg cgatctgctt cagggtgggc ttcttcttct gcttgaacac gttctcgatg
     1021 atctggaact tctcg
//
