LOCUS       sequence-106205-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A single vector AAV-Cas9 system containing Cas9 from Staphylococcus 
            aureus (SaCas9) and its sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B,
            Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F
  TITLE     In vivo genome editing using Staphylococcus aureus Cas9.
  JOURNAL   Nature. 2015 Apr 1. doi: 10.1038/nature14299.
  PUBMED    25830891
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Apr 1. doi: 10.1038/nature14299."
COMMENT     Sequence Label: 
            pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(264..284)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      287..296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(315..724)
                     /label=TBG promoter
                     /note="promoter from the human thyroxine-binding globulin 
                     gene (Yan et al., 2012)"
ORIGIN
        1 ctgtggtcgg tcagcaggtt gtagtcgaac agcagcttct tcactctctg gattctatgc
       61 cgcctccgcc gcttcagcct tctggcgcct ctcttgctcc gcctgccctc gttgttttcc
      121 acgttggcct ctttgaacag ccgcacgccg gcatcgatca cgtcccgtgt ctcgtagtcg
      181 atgatgccgt agcccacgct ggtgatgccg atgtccaggc ccaggatgta gttccgcttg
      241 gctgctggga ctccgtggat accgaccttc cgcttcttct ttggggccat ggtggcaccg
      301 gtatctttcc atttttatag catgtcctgt attgcaaaac tagagcactc ttagttataa
      361 attaacccca gcattaaccc tgggatggta ttttcaaagc tgatgtacaa aaccaaggcc
      421 aagaggaaca aagcaaaaaa tgataaggtt tgaactttga gtgattgctt tggctgccag
      481 acccttcatg taaaacaaaa gagaaagagg attgtcagag ctggaggggt ttaagtccat
      541 gctggcaaga gtgtcttcag caggcagaat aggggccata ggcaaaagca ccaagaggca
      601 gcaggaaaaa gttctcacta ttgggccaaa cagcagtgga attggcagtt ttttaaggga
      661 aagttgtaca aaagcagagg ctgggtgatc ctttctaata ggttctgtag aaattataca
      721 tgcattcgca gaggaaatga tgtcaaaggt agcttccagc ccctcgagtc tagaggccgc
      781 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcg
//
