LOCUS       sequence-106209-        9879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of GFP-Mps1 fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Foijer F, Xie SZ, Simon JE, Bakker PL, Conte N, Davis SH, Kregel E, 
            Jonkers J, Bradley A, Sorger PK
  TITLE     Chromosome instability induced by Mps1 and p53 mutation generates 
            aggressive lymphomas exhibiting aneuploidy-induced stress.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13427-32. doi: 
            10.1073/pnas.1400892111. Epub 2014 Sep 2.
  PUBMED    25197064
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400892111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-09-16- 16"; volume: "111"; issue: "37"; pages: 
            "13427-32"
COMMENT     Sequence Label: pRetroX GFP Mps1 wt
FEATURES             Location/Qualifiers
     source          1..9879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1603..1917
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1611..1629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1647..1665
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1682..1700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1718..1736
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1754..1772
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1789..1807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1825..1843
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1890..1914
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(1931..1933,1934..1936,1937..2647)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1931..1933,1934..1936,1937..2647)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1976..1997)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2237..2256)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2584..2605
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5285..5784
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5320..5343)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5717..5736
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5805..6404
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5805..5824)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6301..6321
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6585..7010
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7113..7135)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7271..7291)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7276..7605
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7456..7591
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7627..7646
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7727..7744)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7898..8486)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7978..7997)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8657..9448,9449..9517))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9280..9299
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9518..9622)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9690..9708
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9808..9879,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgaggccctt tcgtcttcac tcgagtttac tccctatcag
     1621 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat gtcgagttta
     1681 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
     1741 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg
     1801 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg
     1861 cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     1921 agaaggatct atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1981 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     2041 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     2101 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     2161 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     2221 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2281 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2341 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     2401 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     2461 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2521 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     2581 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     2641 gtacaaggga ggacccggag gaggatccat ggaatccgag gatttaagtg gcagagaatt
     2701 gacaattgat tccataatga acaaagtgag agacattaaa aataagttta aaaatgaaga
     2761 ccttactgat gaactaagct tgaataaaat ttctgctgat actacagata actcgggaac
     2821 tgttaaccaa attatgatga tggcaaacaa cccagaggac tggttgagtt tgttgctcaa
     2881 actagagaaa aacagtgttc cgctaagtga tgctctttta aataaattga ttggtcgtta
     2941 cagtcaagca attgaagcgc ttcccccaga taaatatggc caaaatgaga gttttgctag
     3001 aattcaagtg agatttgctg aattaaaagc tattcaagag ccagatgatg cacgtgacta
     3061 ctttcaaatg gccagagcaa actgcaagaa atttgctttt gttcatatat cttttgcaca
     3121 atttgaactg tcacaaggta atgtcaaaaa aagtaaacaa cttcttcaaa aagctgtaga
     3181 acgtggagca gtaccactag aaatgctgga aattgccctg cggaatttaa acctccaaaa
     3241 aaagcagctg ctttcagagg aggaaaagaa gaatttatca gcatctacgg tattaactgc
     3301 ccaagaatca ttttccggtt cacttgggca tttacagaat aggaacaaca gttgtgattc
     3361 cagaggacag actactaaag ccaggttttt atatggagag aacatgccac cacaagatgc
     3421 agaaataggt taccggaatt cattgagaca aactaacaaa actaaacagt catgcccatt
     3481 tggaagagtc ccagttaacc ttctaaatag cccagattgt gatgtgaaga cagatgattc
     3541 agttgtacct tgttttatga aaagacaaac ctctagatca gaatgccgag atttggttgt
     3601 gcctggatct aaaccaagtg gaaatgattc ctgtgaatta agaaatttaa agtctgttca
     3661 aaatagtcat ttcaaggaac ctctggtgtc agatgaaaag agttctgaac ttattattac
     3721 tgattcaata accctgaaga ataaaacgga atcaagtctt ctagctaaat tagaagaaac
     3781 taaagagtat caagaaccag aggttccaga gagtaaccag aaacagtggc aatctaagag
     3841 aaagtcagag tgtattaacc agaatcctgc tgcatcttca aatcactggc agattccgga
     3901 gttagcccga aaagttaata cagagcagaa acataccact tttgagcaac ctgtcttttc
     3961 agtttcaaaa cagtcaccac caatatcaac atctaaatgg tttgacccaa aatctatttg
     4021 taagacacca agcagcaata ccttggatga ttacatgagc tgttttagaa ctccagttgt
     4081 aaagaatgac tttccacctg cttgtcagtt gtcaacacct tatggccaac ctgcctgttt
     4141 ccagcagcaa cagcatcaaa tacttgccac tccacttcaa aatttacagg ttttagcatc
     4201 ttcttcagca aatgaatgca tttcggttaa aggaagaatt tattccatat taaagcagat
     4261 aggaagtgga ggttcaagca aggtatttca ggtgttaaat gaaaagaaac agatatatgc
     4321 tataaaatat gtgaacttag aagaagcaga taaccaaact cttgatagtt accggaacga
     4381 aatagcttat ttgaataaac tacaacaaca cagtgataag atcatccgac tttatgatta
     4441 tgaaatcacg gaccagtaca tctacatggt aatggagtgt ggaaatattg atcttaatag
     4501 ttggcttaaa aagaaaaaat ccattgatcc atgggaacgc aagagttact ggaaaaatat
     4561 gttagaggca gttcacacaa tccatcaaca tggcattgtt cacagtgatc ttaaaccagc
     4621 taactttctg atagttgatg gaatgctaaa gctaattgat tttgggattg caaaccaaat
     4681 gcaaccagat acaacaagtg ttgttaaaga ttctcaggtt ggcacagtta attatatgcc
     4741 accagaagca atcaaagata tgtcttcctc cagagagaat gggaaatcta agtcaaagat
     4801 aagccccaaa agtgatgttt ggtccttagg atgtattttg tactatatga cttacgggaa
     4861 aacaccattt cagcagataa ttaatcagat ttctaaatta catgccataa ttgatcctaa
     4921 tcatgaaatt gaatttcccg atattccaga gaaagatctt caagatgtgt taaagtgttg
     4981 tttaaaaagg gacccaaaac agaggatatc cattcctgag ctcctggctc atccatatgt
     5041 tcaaattcaa actcatccag ttaaccaaat ggccaaggga accactgaag aaatgaaata
     5101 tgttctgggc caacttgttg gtctgaattc tcctaactcc attttgaaag ctgctaaaac
     5161 tttatatgaa cactatagtg gtggtgaaag tcataattct tcatcctcca agacttttga
     5221 aaaaaaaagg ggaaaaaaat gagcggccgc gccggctcta gatcgcgaac gcgtgaattc
     5281 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     5341 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     5401 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     5461 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     5521 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     5581 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     5641 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     5701 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     5761 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
     5821 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     5881 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     5941 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     6001 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     6061 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     6121 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     6181 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     6241 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     6301 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     6361 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     6421 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc ggcgtctcga gatatcagtg
     6481 gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca
     6541 tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     6601 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     6661 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagggtc gaccctagag
     6721 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     6781 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     6841 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
     6901 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     6961 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg
     7021 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc
     7081 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     7141 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     7201 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtag
     7261 atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7321 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7381 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7441 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7501 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7561 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tactggctta
     7621 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     7681 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     7741 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7801 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7861 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7921 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7981 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8041 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8101 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8161 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8221 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8281 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     8341 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8401 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8461 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8521 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8581 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8641 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8701 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8761 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8821 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8881 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8941 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     9001 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9061 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9121 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9181 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9241 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     9301 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9361 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9421 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9481 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9541 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9601 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     9661 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     9721 aagaattagc ttggccattg catacgttgt atccatatca taatatgtac atttatattg
     9781 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
     9841 caattacggg gtcattagtt catagcccat atatggagt
//
