LOCUS       sequence-106240-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human immunoglobulin epsilon antibody expression vector (lambda 
            light chain) without variable regions. Enables Colony-PCR screening 
            of false-positive (PCR vector template) colonies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, 
            Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
  TITLE     A tool kit for rapid cloning and expression of recombinant 
            antibodies.
  JOURNAL   Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
  PUBMED    25073855
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-07-30"; volume: "4:5885. doi"; pages: " 
            10.1038/srep05885"
COMMENT     Sequence Label: pVITRO1-dV-IgE/lambda
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    197..318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(234..253)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     288..307
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
       61 tcacgttaag ggattttggt catggctagt taattaacat ttccatacca catttgtaga
      121 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
      181 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
      241 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
      301 actcatcaat gtatcttatc atgtctggcc agctaatcat ttaccgggat ttacagacac
      361 cgctcgctgg acggtctgtg aggggctcgc tgcctcatgg actgcacggc agatgaactc
      421 atctttctgc tcccattcgg ccctggtcac ctccaggcgg ctgaagacga agaagccgga
      481 gcccttggtc ttgcggggct gcgtcgtgct gtgccgggcg tccgggagct gcacctcgtt
      541 gtgcagccac tgcaccgaga tgtcctcagg catgaagttc tggatcaggc aggcgagggt
      601 gcgcttgtcc cggctccccg gccactccgg cgtcgcaaac gcatagactt ccggggcagc
      661 acgcgggccg ctggtcttgg tcgtggaccg catgagggcc ctgggcaggt gggggtgggt
      721 caccctgcac tggtaggtct ccccctcgat ccagtctcgg gtgcccaccg gcagggtgga
      781 cgtgacggtt aacgtgccat tgcgctgctt ctcctccttt ctggtggagt ggttcacagg
      841 cttcccactg gc
//
