LOCUS       sequence-106243-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_5
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..32
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(63..112)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     terminator      complement(181..208)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(224..295)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(518..535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(732..751)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctcctttac tcatctagta tttctcctct ttctctagat taaacaaaat tatttgtaga
       61 ggctgtttcg tcctcacgga ctcatcagac cggaaagcac atccggtgac agctcagatc
      121 agggtgcgca agttgtcaac gctcccagga gagttatcga cttgcgtatt agggtataaa
      181 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagagcg ttcaccgaca
      241 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
      301 tggctagggc ccatacccaa aaaaaaaccc cgcccctgac agggcggggt tttttttagg
      361 tagagaccca cgaggcagac ctcagcgcta gcggagtgta tactggctta ctatgttggc
      421 actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc
      481 gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct
      541 cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag
      601 atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag
      661 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc
      721 gacaggacta taaagatacc aggcgtttcc cc
//
