LOCUS       sequence-106257-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of Ngn1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Blanchard JW, Eade KT, Szucs A, Lo Sardo V, Tsunemoto RK, Williams 
            D, Sanna PP, Baldwin KK
  TITLE     Selective conversion of fibroblasts into peripheral sensory neurons.
  JOURNAL   Nat Neurosci. 2015 Jan;18(1):25-35. doi: 10.1038/nn.3887. Epub 2014 
            Nov 24.
  PUBMED    25420069
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3887"; 
            journalName: "Nat Neurosci"; date: "2015-01"; volume: "18"; issue: 
            "1"; pages: "25-35"
COMMENT     Sequence Label: Teto Ngn1
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..84
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     69..85
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    88..676
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             194..205
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     603..623
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gccaggcaca atcagcattg gtagctgctg tattgctact tgtgattgct ccatgttttt
       61 ctaggtctcg aggtcgacgg tatcgatgcg gggaggcggc ccaaagggag atccgactcg
      121 tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag
      181 gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc
      241 aggtggcaac acaggcgagc agccaaggaa aggacgatga tttccccgac aacaccacgg
      301 aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga
      361 gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg
      421 tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt
      481 gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg
      541 agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc
      601 cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt
      661 aatccagagg ttgattatcg ataagcttga tatcgaattg ttaacggatc cgctagcttc
      721 gaagaattca cttgggggta agtgtggccc ccggaggctt cagccagttc cccatctatt
      781 attgctctta gactggggga ggaagaaagt attgatgttg ccttacaaag gcctagtggt
      841 atgggatgaa acagggcgtc gtgtggagca ggtctttggg caggccagga aaggagaaaa
      901 ggggatcgcc cgggccatag gtgaagtctt ctgaagccga gggactactg gggtcagaga
      961 gtggtgatgc cacagtggcg cag
//
