LOCUS       sequence-106269-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 493, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mWasabi-Mito-7
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..112
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      140..149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             146..856
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcatg tccgtcctga cgccgctgct
       61 gctgcggggc ttgacaggct cggcccggcg gctcccagtg ccgcgcgcca agatccattc
      121 gttgggggat ccaccggtcg ccaccatggt gagcaagggc gaggagacca caatgggcgt
      181 aatcaagccc gacatgaaga tcaagctgaa gatggagggc aacgtgaatg gccacgcctt
      241 cgtgatcgag ggcgagggcg agggcaagcc ctacgacggc accaacacca tcaacctgga
      301 ggtgaaggag ggagcccccc tgcccttctc ctacgacatt ctgaccaccg cgttcagtta
      361 cggcaacagg gccttcacca agtaccccga cgacatcccc aactacttca agcagtcctt
      421 ccccgagggc tactcttggg agcgcaccat gaccttcgag gacaagggca tcgtgaaggt
      481 gaagtccgac atctccatgg aggaggactc cttcatctac gagatacacc tcaagggcga
      541 gaacttcccc cccaacggcc ccgtgatgca gaaggagacc accggctggg acgcctccac
      601 cgagaggatg tacgtgcgcg acggcgtgct gaagggcgac gtcaagatga agctgctgct
      661 ggagggcggc ggccaccacc gcgttgactt caagaccatc tacagggcca agaaggcggt
      721 gaagctgccc gactatcact ttgtggacca ccgcatcgag atcctgaacc acgacaagga
      781 ctacaacaag gtgaccgttt acgagatcgc cgtggcccgc aactccaccg acggcatgga
      841 cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc acatt
//
