LOCUS       sequence-106275-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Note- This plasmid contains Keratin 18. Localization: Cytokeratin, 
            Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Keratin-17
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(142..819)
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     regulatory      816..825
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgcataacaa gtttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
       61 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
      121 tagagtcgcg gccgcctatc attacttggc ctgcctcggc agctcagaat gcgcttcggc
      181 gtgctcatgc agattaacat tactgtaatc tttgtcgtgg cttttaatct caatgtggtg
      241 gtccacaaag tgatagtctg gcaactggac aaccttctta gctttataag tagttttgaa
      301 gtcacatcgg taatggccac ctccttcaag cgacagagcc atgttaacat cacccttcag
      361 cactccatca cgcacataca atttctcagt ggatggctcc catttcacag tcctcttctg
      421 cataactgga ccattggcag gaaagttcac accatcaaat cgaatttcat agatataaca
      481 gtcaccatcc agggttatgt cgtttgtcgc gttacaaatg cccccgtctt cgtaattcat
      541 gcttcgttcc caagagtagc cctcaggaaa cgactgcttg aaatagtcta ctatattttc
      601 tgggtatttg gcgaataccc tgttgccgta acagaacaca gttgtcaaga tgtcataggc
      661 gaaaggcaga ggtccgcctt ctttgacttt aaggtccata ctctgttttc cctcgaaagg
      721 cttcccaagg ccaactcctt caatcgcgaa cgggtgtcca tttacagcgc cttccatacg
      781 cagcttgatc ttcatgtctg gtttaatcac actcaccatg gtggcgaccg gtggat
//
