LOCUS       sequence-106279-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, 
            under EF1a promoter, in AAV virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  TITLE     pAAV-FLEX-C-RG
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-FLEX-C-RG
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    49..82
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    145..178
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tttcaggtgt cgtgaggtac cggatcctct agagccgcca ccatggccat aacttcgtat
       61 aaagtatcct atacgaagtt atatcaaaat aggaagacca atgcttcacc atcgacccga
      121 attgccaagc atcaccatcg acccataact tcgtataatg tatgctatac gaagttatac
      181 ttcaactagt tcacagtctg gtctcgccgc cgctcttgtg gctctcccag ctgctgatga
      241 tcttgccgct ctggggggtc acgctcacct ctctgccggt gcctctcagg ttgtgctggg
      301 tgggctcgct tctgttcact cttctgcagc aggtcatcag gaagatgatc agcatcaggg
      361 cggtcagggc gccggcgctc agcagcacgt acttgcccca gttgggcagg cccaggtcca
      421 cgccgctcac ctggttgtgc acgtcgggca ggtgcacctc cacgaagtcc tcggcctcgt
      481 cgccgtcctt gaacacggtg ctggggtcgg ccagggggtg caccaggggg atcacgctgc
      541 tctccagcag ctccatgtgc tgctgcagca ggctgctctg catctcgggg atcagcacgt
      601 tgccgtcggg gcccaggatg atgccgttga agaacacgcc gttcacgtgg gggtggcatc
      661 tgccgcccac tctcaggcag cccttgctgg gcaggatctc gttccaggtt ctcacgctct
      721 tgtagtgggc gtcggcctcc atcagggtct tgttgaagat ggtgtaggcc ttgccgaagc
      781 cgggcaccag ctttctcagg tggctcagtc ttctgaagct cacgctcttg gtggtcatga
      841 tgctctccag ggcgtccagg cactcctctc tctttctcac cagctcctcc accaccaggt
      901 gctcgatctc gtcgcttctg aagtcgtgca ggttcaccag ctt
//
