LOCUS       sequence-106285-        1497 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Voltage sensing domain of Ciona with the D129S/R168A/D186S/R229I 
            mutations fused to super ecliptic pHlorin A227D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
  TITLE     Combinatorial mutagenesis of the voltage-sensing domain enables the 
            optical resolution of action potentials firing at 60 Hz by a 
            genetically encoded fluorescent sensor of membrane potential.
  JOURNAL   J Neurosci. 2015 Jan 7;35(1):372-85. doi: 
            10.1523/JNEUROSCI.3008-14.2015.
  PUBMED    25568129
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3008-14"; journalName: "J Neurosci"; date: 
            "2015-01-7- 7"; volume: "35"; issue: "1"; pages: "372-85"
COMMENT     Sequence Label: CC4
FEATURES             Location/Qualifiers
     source          1..1497
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             781..1497
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTADGITHGMDELYK"
     primer_bind     complement(811..839)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1434..1454
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctagcgcca ccatggaggg attcgacggt tcagatttta gtcctccagc tgatttagtt
       61 ggcgttgacg gtgcagtcat gcggaacgtc gttgacgtca cgataaatgg tgacgtcact
      121 gctccgccga aagctgcgcc aagaaaaagt gaatcggtaa agaaagttca ttggaatgat
      181 gtagaccaag gaccgagtga aaaaccagag acaagacagg aggaacgaat agatataccc
      241 gagatttcag gtctatggtg gggcgagaat gaacatggag tggacgatgg gagaatggag
      301 atacctacta ctggtgtagg tcgcgtccag tttcggatcc gagcagtgat tgatcatcta
      361 gggatgcgag tctttggagt cttcctaatt ttcttgagca tcatcctcat gatcattgat
      421 ctcagtctta tcgataaaag tgaatcttca caatcctttt atgacgggat ggccttggct
      481 ctttcttgtt atttcatgct ggacttagga ttagccatat ttgcctacgg gcccaagctt
      541 ttcttcacca acccctggga ggttgcttcc ggtttgatta tcgttgtcac attcgtcgtc
      601 acgatatttt acactgtgtt agatgaatac ggtaccgaaa caggagccga tggtttgggg
      661 cggttggttg tgttggccag attgctgcgt gtggttatct tagcaagaat attttattcc
      721 caccaacaaa tgaaggcttc aagcagaaga acaatatcac agaacaaaag aagatatcgc
      781 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      841 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      901 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      961 gtcactactt taacttatgg tgttcaatgc ttttcaagat acccagatca tatgaaacgg
     1021 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
     1081 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
     1141 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
     1201 ttggaataca actataacga tcaccaggtg tacatcatgg cagacaaaca aaagaatgga
     1261 atcaaagcta acttcaaaat tagacacaac attgaagatg gaggcgttca actagcagac
     1321 cattatcaac aaaatactcc aattggcgat gggcccgtcc ttttaccaga caaccattac
     1381 ctgtttacaa cttctactct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     1441 cttgagtttg taacagctga tgggattaca catggcatgg atgaactata caaatga
//
