LOCUS       sequence-106300-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             54..707
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga
       61 gtgtgattaa accagagatg aagatgaggt actacatgga cggctccgtc aatgggcatg
      121 agttcacaat tgaaggtgaa ggcacaggca gaccttacga gggacatcaa gagatgacac
      181 tacgcgtcac aatggccgag ggcgggccaa tgcctttcgc gtttgactta gtgtcacacg
      241 tgttctgtta cggccacaga gtatttacta aatatccaga agagatacca gactatttca
      301 aacaagcatt tcctgaaggc ctgtcatggg aaaggtcgtt ggagttcgaa gatggtgggt
      361 ccgcttcagt cagtgcgcat ataagcctta gaggaaacac cttctaccac aaatccaaat
      421 ttactggggt taactttcct gccgatggtc ctatcatgca aaaccaaagt gttgattggg
      481 agccatcaac cgagaaaatt actgccagcg acggagttct gaagggtgat gttacgatgt
      541 acctaaaact tgaaggaggc ggcaatcaca aatgccaaat gaagactact tacaaggcgg
      601 caaaagagat tcttgaaatg ccaggagacc attacatcgg ccatcgcctc gtcaggaaaa
      661 ccgaaggcaa cattactgag caggtagaag atgcagtagc tcatagctcc ggactcagat
      721 ctcgagctca agcttcgatg gcgactgcga cccccgtgcc gccgcggatg ggcagccgcg
      781 ctggcggccc caccacgccg ctgagcccca cgcgcctgtc gcggctccag gagaaggagg
      841 agctgcgcga gctcaatgac cggctggcgg tgtacatcga caaggtgcgc agcctgg
//
