LOCUS       sequence-106305-         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway vector for generating cyan fluorescent protein fusions in 
            fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin 
            MM, Farman M
  TITLE     pFPL vectors for high-throughput protein localization in fungi: 
            detecting cytoplasmic accumulation of putative effector proteins.
  JOURNAL   Mol Plant Microbe Interact. 2014 Nov 12.
  PUBMED    25390188
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2014 Nov 12."
COMMENT     Sequence Label: pFPL-Cg
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(85..91,92..109,110..115))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(239..263)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttaacttc cttagctcct gaaaatctcg
       61 acggatccta actcaaaatc cacacattat acgagccgga agcataaagt gtaaaacctg
      121 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
      181 ctgtttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
      241 tcagcttttt tgtacaaact tgtgatccag atgtctatcg ttcgtaagct tataaatcac
      301 tagtgaattc gcggccgcct gcaggtcgac catatgggag agctctgtac agtgaccggt
      361 gactctttct ggcatgcgga gagacggacg gacgcagaga gaagggctga gtaataagcg
      421 ccactgcgcc agacagctct ggcggctctg aggtgcagtg gatgattatt aatccgggac
      481 cggccgcccc tccgccccga agtggaaagg ctggtgtgcc cctcgttgac caagaatcta
      541 ttgcatcatc ggagaatatg gagcttcatc gaatcaccgg cagtaagcga aggagaatgt
      601 gaagccaggg gtgtatagcc gtcggcgaaa ta
//
