LOCUS       sequence-106306-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase protein in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V,
            Lebbink RJ, Rehmann H, Geijsen N
  TITLE     Efficient Intracellular Delivery of Native Proteins.
  JOURNAL   Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028.
  PUBMED    25910214
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.03"; journalName: "Cell"; date: "2015-04-23- 
            23"; volume: "161"; issue: "3"; pages: "674-690"
COMMENT     Sequence Label: CRE recombinase
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(10..15,16..32,33..38)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(213..260)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     256..274
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
      241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
      301 cgggctttgt tagcagccgg atcctcagtc gccatcttcc agcagacgca ccatcgcacc
      361 cgtttcagag tccaggttac gaatatagtt cataacaata ttcacgttcg tccaaccgcc
      421 ggcctgcata atttccggga tagagacacc tgcacgtgcc atatcacgtg ctgcaccaac
      481 acgagctgag tgaccggacc atgccaggta acgctgaccg ctgtcatctt tcgcgccata
      541 aatcaggcga tgggtggctt cgaagatgcc ttccagcgca cgcgtactca gctgagaggt
      601 agcgctcgga gctgcaacac catttttgcg cacacgacaa aacaggtaat tgttcgggtc
      661 atctgcgacg cccgaaactg aaatccagcg ttccaccagt ttcgtgacac ccagtgacag
      721 ggctttttcg acgcccgcgg tggaaaccag cgttttggta cggccgatgt gaatcagcat
      781 gcgaccgccg tccgtacggc tgatatcttt cacgcgaata cgagcgattt ctgcaatgcg
      841 cagcagggtg ttataagcaa tacccaggaa tgccagattg cggatgtcct ggcaacgatc
      901 actgttttcc atcagagagc gaacttggtc gaaatcggta cgttcaaaag ccagtgcctg
      961 tttagcgcgt tcgcctgcat ccacgttttc tttacgaatg cgacgcatca ccagactgac
     1021 ggcattggaa tcactcgga
//
