LOCUS       sequence-106310-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Cre recombinase reporter; 
            EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked 
            RFP-STOP cassette and switches fluorescence from red (RFP) to green 
            (GFP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V,
            Lebbink RJ, Rehmann H, Geijsen N
  TITLE     Efficient Intracellular Delivery of Native Proteins.
  JOURNAL   Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028.
  PUBMED    25910214
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.03"; journalName: "Cell"; date: "2015-04-23- 
            23"; volume: "161"; issue: "3"; pages: "674-690"
COMMENT     Sequence Label: Cre Reporter
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..79)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             114..791
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(294..314)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     512..531
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     706..729
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(816..849)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      900..909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttcaggtgt cgtgaagcgg ccgcctagtc cagtgtggtg gaattataac ttcgtatagc
       61 atacattata cgaagttata attctgcaga tatccagcac agtggcggcc accatggata
      121 gcactgagaa cgtcatcaag cccttcatgc gcttcaaggt gcacatggag ggctccgtga
      181 acggccacga gttcgagatc gagggcgagg gcgagggcaa gccctacgag ggcacccaga
      241 ccgccaagct gcaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
      301 cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc cccgactaca
      361 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      421 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg caccttcatc taccacgtga
      481 agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaag actctgggct
      541 gggagccctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccaca
      601 aggcgctgaa gctgaagggc ggcggccact acctggtgga gttcaagtca atctacatgg
      661 ccaagaagcc cgtgaagctg cccggctact actacgtgga ctccaagctg gacatcacct
      721 cccacaacga ggactacacc gtggtggagc agtacgagcg cgccgaggcc cgccaccacc
      781 tgttccagta gagctagctg aataaggccg ctcgaataac ttcgtatagc atacattata
      841 cgaagttatt cgagtctaga gggcccgtta gatctctcga ggttaacgaa ttcgactacg
      901 ccaccatggt gagcaagg
//
