LOCUS       sequence-106312-         430 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Cre recombinase reporter; 
            EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked 
            RFP-STOP cassette and switches fluorescence from red (RFP) to green 
            (GFP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 430)
  AUTHORS   D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V,
            Lebbink RJ, Rehmann H, Geijsen N
  TITLE     Efficient Intracellular Delivery of Native Proteins.
  JOURNAL   Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028.
  PUBMED    25910214
REFERENCE   2  (bases 1 to 430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.03"; journalName: "Cell"; date: "2015-04-23- 
            23"; volume: "161"; issue: "3"; pages: "674-690"
COMMENT     Sequence Label: Cre Reporter
FEATURES             Location/Qualifiers
     source          1..430
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(191..212)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctcgtcaag aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat
       61 ggtggaaaat aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt
      121 aacgttaggg gggggggagg gatccttact tgtacagctc gtccatgccg agagtgatcc
      181 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
      241 tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
      301 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
      361 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
      421 ggctgttgta
//
