LOCUS       sequence-106316-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian vesicle leakage reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V,
            Lebbink RJ, Rehmann H, Geijsen N
  TITLE     Efficient Intracellular Delivery of Native Proteins.
  JOURNAL   Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028.
  PUBMED    25910214
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.03"; journalName: "Cell"; date: "2015-04-23- 
            23"; volume: "161"; issue: "3"; pages: "674-690"
COMMENT     Sequence Label: mAG-GAL3
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             26..703
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             26..703
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
ORIGIN
        1 tttcaggtgt cgtgaagcgg ccgccatggt gagcgtgatc aagcccgaga tgaagatcaa
       61 gctgtgcatg aggggcaccg tgaacggcca caacttcgtg atcgagggcg agggcaaggg
      121 caacccctac gagggcaccc agatcctgga cctgaacgtg accgagggcg cccccctgcc
      181 cttcgcctac gacatcctga ccaccgtgtt ccagtacggc aacagggcct tcaccaagta
      241 ccccgccgac atccaggact acttcaagca gaccttcccc gagggctacc actgggagag
      301 gagcatgacc tacgaggacc agggcatctg caccgccacc agcaacatca gcatgagggg
      361 cgactgcttc ttctacgaca tcaggttcga cggcaccaac ttccccccca acggccccgt
      421 gatgcagaag aagaccctga agtgggagcc cagcaccgag aagatgtacg tggaggacgg
      481 cgtgctgaag ggcgacgtga acatgaggct gctgctggag ggcggcggcc actacaggtg
      541 cgacttcaag accacctaca aggccaagaa ggaggtgagg ctgcccgacg cccacaagat
      601 cgaccacagg atcgagatcc tgaagcacga caaggactac aacaaggtga agctgtacga
      661 gaacgccgtg gccaggtact ccatgctgcc cagccaggcc aagggaggag gaggatctgc
      721 agacaatttt tcgctccatg atgcgttatc tgggtctgga aacccaaacc ctcaaggatg
      781 gcctggcgca tgggggaacc agcctgctgg ggcagggggc tacccagggg cttcctatcc
      841 tggggcctac cccgggcagg cacccccagg ggcttatcct ggacaggcac ctccaggcgc
      901 ctaccctgga gcacctggag cttatcccgg agcacctgca cctggagtct acccagggcc
      961 acccagcggc cctggggcct acccatcttc tggacagcca agtgccaccg gagcctaccc
     1021 tgccactggc ccctatggcg cc
//
