LOCUS       sequence-106322-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SP-Cas9 protein in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V,
            Lebbink RJ, Rehmann H, Geijsen N
  TITLE     Efficient Intracellular Delivery of Native Proteins.
  JOURNAL   Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028.
  PUBMED    25910214
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.03"; journalName: "Cell"; date: "2015-04-23- 
            23"; volume: "161"; issue: "3"; pages: "674-690"
COMMENT     Sequence Label: SP-Cas9
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaatcagacc acgcaaaaag gccagaaaaa ctcgcgtgaa cgcatgaaac gcattgaaga
       61 aggtatcaaa gaactgggca gccagattct gaaagaacat ccggtcgaaa acacccagct
      121 gcaaaatgaa aaactgtacc tgtattacct gcaaaatggt cgtgacatgt atgtggatca
      181 ggaactggac atcaaccgcc tgtctgacta tgatgtcgac cacattgtgc cgcagagctt
      241 tctgaaagac gattctatcg ataacaaagt tctgacccgt agtgataaaa accgcggcaa
      301 aagcgacaat gtcccgtctg aagaagttgt gaagaaaatg aaaaactact ggcgtcaact
      361 gctgaatgcg aaactgatta cgcagcgtaa attcgataac ctgaccaaag cggaacgcgg
      421 cggtctgtcc gaactggata aagccggttt tatcaaacgt caactggttg aaacccgcca
      481 gattacgaaa catgtcgccc agatcctgga ttcacgcatg aacacgaaat acgacgaaaa
      541 cgataaactg atccgtgaag tcaaagtgat caccctgaaa agtaaactgg tttccgattt
      601 ccgtaaagac tttcagttct acaaagtccg cgaaattaac aattaccatc acgcacacga
      661 tgcttatctg aatgcagtgg ttggtaccgc tctgatcaaa aaatatccga aactggaaag
      721 cgaatttgtg tatggcgatt acaaagtcta tgacgtgcgc aaaatgattg cgaaatccga
      781 acaggaaatc ggcaaagcga ccgccaaata ctttttctat tcaaacatca tgaacttttt
      841 caaaaccgaa attacgctgg caaatggtga aattcgtaaa cgcccgctga tcgaaaccaa
      901 cggtgaaacg ggcgaaattg tgtgggataa aggccgtgac ttcgcgaccg ttcgcaaagt
      961 cctgtcgatg ccgcaagtga atatcgtgaa gaaaaccgaa gtgcagacgg gcggtttt
//
